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Merck
CN

901765

Sigma-Aldrich

Dde biotin-azide

Synonym(s):

rel-(3aR,4R,6aS)-N-[19-Azido-15-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3,6,9,12-tetraoxa-16-azanonadec-1-yl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide

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CN¥1,434.82
5 G
CN¥5,020.05

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1 G
CN¥1,434.82
5 G
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About This Item

Empirical Formula (Hill Notation):
C32H53N7O8S
CAS Number:
Molecular Weight:
695.87
MDL number:
UNSPSC Code:
12352125
NACRES:
NA.22

CN¥1,434.82


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form

liquid

reaction suitability

reaction type: click chemistry

storage temp.

−20°C (−15°C to −25°C)

SMILES string

S1[C@H]([C@H]3NC(=O)N[C@H]3C1)CCCCC(=O)NCCOCCOCCOCCOCCC(=C2C(=O)CC(CC2=O)(C)C)NCCCN=[N+]=[N-]

InChI

1S/C32H53N7O8S/c1-32(2)20-25(40)29(26(41)21-32)23(34-9-5-10-36-39-33)8-12-44-14-16-46-18-19-47-17-15-45-13-11-35-28(42)7-4-3-6-27-30-24(22-48-27)37-31(43)38-30/h24,27,30,34H,3-22H2,1-2H3,(H,35,42)(H2,37,38,43)/t24-,27-,30-/m0/s1

InChI key

HUAWNOKWZYCBQA-LFERIPGTSA-N

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This Item
OGS624OGS620OGS628
bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide tag location

C-terminal

peptide tag location

N-terminal

peptide tag location

N-terminal

peptide tag location

C-terminal

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

mol wt

size 5897 bp

mol wt

size 5913 bp

mol wt

size 4568 bp

mol wt

size 5923 bp

Application

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a hydrazine-cleavable Dde linker. Typically >90% of captured biomolecules can be efficiently released.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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