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Key Documents

Safety Information

17975

Supelco

Ninhydrin reagent according to Stahl

for TLC derivatization

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50 ML
CN¥889.71

CN¥889.71

Available to ship onApril 27, 2025Details

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50 ML
CN¥889.71

About This Item

UNSPSC Code:
41116105
NACRES:
NA.22

CN¥889.71

Available to ship onApril 27, 2025Details

Request a Bulk Order

Quality Level

100

form

liquid

technique(s)

thin layer chromatography (TLC): suitable

impurities

1-butanol
 acetic acid

density

0.823 g/mL at 20 °C

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Hellma® absorption cuvettes, standard cells, Macro

material

Suprasil® quartz

material

Suprasil® quartz

material

Suprasil® quartz

material

Suprasil® 300 quartz

manufacturer/tradename

Hellma 110-QS

manufacturer/tradename

Hellma (100-QS)

manufacturer/tradename

Hellma (100-QS)

manufacturer/tradename

Hellma 100-QX

description

2 polished windows

description

2 polished windows

description

2 polished windows

description

2 polished windows

parameter

200-2,500 nm spectral range

parameter

200-2500 nm spectral range

parameter

200-2500 nm spectral range

parameter

200-3500 nm spectral range

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

UV/Vis spectroscopy: suitable, photometry: suitable

technique(s)

UV/Vis spectroscopy: suitable, photometry: suitable

technique(s)

UV/Vis spectroscopy: suitable, photometry: suitable

H × W × D

46 mm × 12.5 mm × 12.5 mm

H × W × D

45 mm × 12.5 mm × 3.5 mm

H × W × D

45 mm × 12.5 mm × 4.5 mm

H × W × D

45 mm × 12.5 mm × 4.5 mm

General description

Ninhydrin reagent is mostly used for the detection of amino acids.[1] It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product.[2] Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.[3]

Application

1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid
Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.[3]

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

93.2 °F - closed cup

Flash Point(C)

34 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCF2980
    BCBV2843

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    Thin Layer Chromatography in Phytochemistry.
    Hajnos WM
    Science, 312-312 (2008)
    M K Gaitonde
    The Biochemical journal, 104(2), 627-633 (1967-08-01)
    1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring
    B Starcher
    Analytical biochemistry, 292(1), 125-129 (2001-04-26)
    Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature
    Sylvia Kristyanto et al.
    Journal of microbiology (Seoul, Korea), 56(5), 317-323 (2018-05-04)
    A novel, aerobic, Gram-stain-negative, non-motile, non-spore forming, rod-shaped bacterium, designated strain Dol 15-39T, was isolated from a seawater sample near Geoje Island in the South Sea, Republic of Korea. The strain was found to be oxidase-negative and catalase-positive. The isolate

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