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151173

Sigma-Aldrich

Ninhydrin

ACS reagent

Synonym(s):

1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate

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10 G
CN¥626.68
25 G
CN¥634.47
100 G
CN¥3,511.98

CN¥626.68

Estimated to ship onApril 27, 2025Details

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10 G
CN¥626.68
25 G
CN¥634.47
100 G
CN¥3,511.98

About This Item

Empirical Formula (Hill Notation):
C9H6O4
CAS Number:
485-47-2
Molecular Weight:
178.14
Beilstein:
1910963
EC Number:
207-618-1
MDL number:
MFCD00003791
UNSPSC Code:
12352100
PubChem Substance ID:
24849098
NACRES:
NA.21

CN¥626.68

Estimated to ship onApril 27, 2025Details

Request a Bulk Order

grade

ACS reagent

Quality Level

200

description

identification and melting point passes test

form

solid

color

white to brownish white, crystals

mp

250 °C (dec.) (lit.)

solubility

H2O: passes test

suitability

passes test for sensitivity to amino acids

functional group

hydroxyl
ketone

SMILES string

OC1(O)C(=O)c2ccccc2C1=O

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1 of 4

This Item
N4876NX0403106762
Ninhydrin ACS reagent

151173

Ninhydrin

Ninhydrin suitable for amino acid detection

N4876

Ninhydrin

Ninhydrin

NX0403

Ninhydrin

Ninhydrin GR for analysis ACS,Reag. Ph Eur

106762

Ninhydrin

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

solubility

H2O: passes test

solubility

ethanol: 100 mg/mL, H2O: 50 mg/mL (with sonication)

solubility

-

solubility

-

form

solid

form

powder

form

-

form

solid

mp

250 °C (dec.) (lit.)

mp

250 °C (dec.) (lit.)

mp

-

mp

250-258 °C (decomposition)

grade

ACS reagent

grade

-

grade

-

grade

ACS reagent

description

identification and melting point passes test

description

-

description

-

description

-

General description

Ninhydrin, a tricyclic 1,2,3-trione,[1] is a useful organic building block.[2] It is an amino acid reagent, which reveals latent fingerprints on porous surfaces like paper, cardboard and raw wood. [3]
It is employed for nitrite detection in various samples, identifying contamination, and is also used as a colorimetric reagent.[4]

Application

Ninhydrin (Triketohydrindene hydrate) may be used as a reagent for the quantification of amino acids and peptides by photometric method[5] and colorimetric method.[6] It may be used to prepare the ninhydrin reagent, by mixing ninhydrin and hydrindantin (reduced form of ninhydrin) in dimethyl sulfoxide. This reagent is used in manual ninhydrin method for the quantitative determination of amino acids.[7]
Used for the detection of free amino groups in amino acids, peptides and proteins.
Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines.[8][9] Reaction with sarcosine or proline gives azomethine ylides.[10]

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCN0054
    0000424005
    0000424005
    0000419535
    0000419535

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    Shao-Ru Wang et al.
    Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(13), 1903770-1903770 (2020-07-17)
    CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome-editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need
    Holden.MH
    To Be a Crime Scene Investigator (2006)
    Tetrahedron, 34, 1285-1285 (1978)
    Gazzetta Chimica Italiana, 123, 137-137 (1993)
    E Curotto et al.
    Analytical biochemistry, 211(2), 240-241 (1993-06-01)
    Chitosans were quantified with ninhydrin, a reagent normally used for recognizing and quantifying amino groups. The reaction was time dependent and there was no effect when different acids were added to the mixture. This method was used to determine the
    View All Related Papers

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