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NAD100-RO

Roche

NAD

Grade I, free acid

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Synonym(s):
β-Nicotinamide adenine dinucleotide hydrate, β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide
Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
53-84-9
Molecular Weight:
663.43 (anhydrous basis)
MDL number:
MFCD00036253
UNSPSC Code:
12352200
PubChem Substance ID:
329818699

Assay

100% (dry weight)

form

lyophilized

mol wt

Mr 663.4

packaging

pkg of 1 g (10127965001)
pkg of 5 g (10127973001)

manufacturer/tradename

Roche

color

off-white

pH

~3.0 (50 mg/mL in water)

λmax

260 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

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General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.
NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

Biochem/physiol Actions

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.
Electron acceptor

Packaging

Packaged by solid weight.

Quality

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Specifications

Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.
This is the common form of NAD.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction
Fernandes PM, et al.
The Biochemical Journal, 476(2), 179-191 (2019)
N J Hughes et al.
Journal of bacteriology, 180(5), 1119-1128 (1998-03-12)
Helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953-3959, 1995)
Chu-Fang Chou et al.
The Journal of biological chemistry, 277(28), 25209-25216 (2002-05-09)
Molecular genetic studies have indicated that alcohol dehydrogenase may be involved in the synthesis of retinoic acid, a hormonal molecule regulating diverse cellular functions at the transcriptional level. Class IV alcohol dehydrogenase (ADH) has been reported to be the most
Jinrui Zhang et al.
Scientific reports, 5, 12846-12846 (2015-08-06)
Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofactors. NADP(H) are critical components
Extracellular beta-nicotinamide adenine dinucleotide (beta-NAD) promotes the endothelial cell barrier integrity via PKA-and EPAC1/Rac1-dependent actin cytoskeleton rearrangement
Umapathy NS, et al.
Journal of Cellular Physiology, 223(1), 215-223 (2010)
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