NAD100-RO
Roche
NAD
Grade I, free acid
Synonym(s):
β-Nicotinamide adenine dinucleotide hydrate, β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide
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About This Item
Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
Molecular Weight:
663.43 (anhydrous basis)
MDL number:
UNSPSC Code:
41106305
PubChem Substance ID:
Recommended Products
Assay
100% (dry weight)
form
lyophilized
mol wt
Mr 663.4
packaging
pkg of 1 g (10127965001)
pkg of 5 g (10127973001)
manufacturer/tradename
Roche
color
off-white
pH
~3.0 (50 mg/mL in water)
λmax
260 nm
shipped in
wet ice
storage temp.
2-8°C
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General description
Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.
Application
NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.
NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.
Biochem/physiol Actions
Electron acceptor
NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.
Packaging
Packaged by solid weight.
Quality
Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)
Specifications
Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)
Preparation Note
Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C
Other Notes
For life science research only. Not for use in diagnostic procedures.
This is the common form of NAD.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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N J Hughes et al.
Journal of bacteriology, 180(5), 1119-1128 (1998-03-12)
Helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953-3959, 1995)
The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction
Fernandes PM, et al.
The Biochemical Journal, 476(2), 179-191 (2019)
Chu-Fang Chou et al.
The Journal of biological chemistry, 277(28), 25209-25216 (2002-05-09)
Molecular genetic studies have indicated that alcohol dehydrogenase may be involved in the synthesis of retinoic acid, a hormonal molecule regulating diverse cellular functions at the transcriptional level. Class IV alcohol dehydrogenase (ADH) has been reported to be the most
SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation
Sarikhani M, et al.
eLife, 7(1), e32952-e32952 (2018)
Jinrui Zhang et al.
Scientific reports, 5, 12846-12846 (2015-08-06)
Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofactors. NADP(H) are critical components
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