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form
lyophilized
packaging
pkg of 1 mg (10223549001)
pkg of 5 mg (11004875001)
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
Histone H1 plays an essential role in maintaining and establishing higher order chromatin structures. The linker histone H1 basically binds to the DNA which is entering and exiting the nucleosomal core particle. All H1 variants in higher eukaryotes comprises of a general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. It has been suggested that each H1 variant has a specific function owing to its tail region which is specifically conserved among species, but differ in variants.
Application
Lysine-rich preparation used as a substrate for protein kinases.
Biochem/physiol Actions
Histone H1 was used as a substrate in kinase assay.
Studies have suggested that histoneH1 might act as a general repressor of transcription.
Quality
Typical analysis: The preparation is electrophoretically homogeneous.
Typical analysis: The preparation is electrophoretically homogeneous.
Sequence
Histone H1 consists of 212 amino acids.
Histone H1 has a very high lysine content (approx. 25%) which contributes to its high basic charge.
Preparation Note
Working concentration: 1 to 5 mg/ml
Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Aqueous solution of Histone H1 is stable at -15 to -25 °C for at least three months.
Avoid repeated freezing and thawing!
Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Aqueous solution of Histone H1 is stable at -15 to -25 °C for at least three months.
Avoid repeated freezing and thawing!
Other Notes
For life science research only. Not for use in diagnostic procedures.
Regulatory Information
监管及禁止进口产品
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Annalisa Izzo et al.
Biological chemistry, 389(4), 333-343 (2008-01-23)
The linker histone H1 binds to the DNA entering and exiting the nucleosomal core particle and has an important role in establishing and maintaining higher order chromatin structures. H1 forms a complex family of related proteins with distinct species, tissue
Boris Kysela et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(6), 1877-1882 (2005-01-27)
DNA nonhomologous end-joining in vivo requires the DNA-dependent protein kinase (DNA-PK) and DNA ligase IV/XRCC4 (LX) complexes. Here, we have examined the impact of histone octamers and linker histone H1 on DNA end-joining in vitro. Packing of the DNA substrate
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