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biological source
bovine (calf) thymus
Quality Level
type
Type III-S
form
powder
concentration
≥60% protein (biuret)
technique(s)
activity assay: suitable
solubility
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
storage temp.
2-8°C
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Application
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
- in Cdk5 activity assay
- in histone H1 kinase assay
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells
Biochem/physiol Actions
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.
Features and Benefits
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Other Notes
Characterized as mainly subgroup f1 by SDS-PAGE
Preparation Note
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
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Scott T
Concise encyclopedia of biochemistry, 265-265 (1988)
P Balsas et al.
Journal of hematology & oncology, 10(1), 80-80 (2017-04-01)
Pharmacological inhibition of B cell receptor (BCR) signaling has recently emerged as an effective approach in a wide range of B lymphoid neoplasms. However, despite promising clinical activity of the first Bruton's kinase (Btk) and spleen tyrosine kinase (Syk) inhibitors
Amit K Dutta et al.
Journal of colloid and interface science, 324(1-2), 55-60 (2008-05-30)
The real-time changes in viscoelasticity of adsorbed poly(L-lysine) (PLL) and adsorbed histone (lysine rich fraction) due to cross-linking by glutaraldehyde and corresponding release of associated water were investigated using a quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total
Mária Čížková et al.
Cells, 8(7) (2019-07-20)
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter
Gyubum Lim et al.
Science advances, 6(6), eaay2669-eaay2669 (2020-02-23)
Homologous recombination is exquisitely activated only during specific cell phases. In the G1 phase, homologous recombination activity is completely suppressed. According to previous reports, the activation of homologous recombination during specific cell phases depends on the kinase activity of cyclin-dependent
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