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Key Documents

Safety Information

SRP0177

Sigma-Aldrich

Histone H3 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

HIST1H3E, Histone H3.1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
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biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

15.4 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

technique(s)

activity assay: suitable

solubility

water: soluble

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sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

turquoise

material

turquoise

material

red

material

-

packaging

pack of 200 ea (2 bags × 100 tips)

packaging

pack of 200 ea (2 bags × 100 tips)

packaging

pack of 500 ea (5 bags × 100 tips)

packaging

pack of 500 ea (5 bags × 100 tips)

availability

available only in (India)

availability

available only in (India)

availability

available only in (India)

availability

available only in (India)

capacity

1-10 mL

capacity

0.5-10 mL

capacity

500-2500 μL

capacity

100-5000 μL

General description

Research area: Cell signaling. The gene HIST1H3E (histone 3, also referred to as H3.1) is mapped to human chromosome 6p22.[1] It is a variant of histone 3 and is also called the replicative histone.[2] HIST1H3E is mainly expressed in the S-phase.[3] In the process of cellular differentiation, HIST1H3E mRNA levels go down as cell division slows down.[4]Human HIST1H3E, GenBank Accession No. NM_003532, amino acids 2-137 (end) with N-terminal His-tag, MW = 15.4kDa, expressed in an Escherichia coli expression system.

Application

Histone H3 full-length human has been used as a substrate in in vitro kinase assay for Aurora-A (AURKA) kinase and Tousled-Like Kinase 2(TLK2)[5]. Histone H3 full-length human has been used in the generation of antibodies in chickens.[6] It has also been used in in vitro methylation assay.[7]

Biochem/physiol Actions

Histones are important for proper organization and modification of chromatin.[8] HIST1H3E (histone 3, also referred to as H3.1) is mainly associated with DNA synthesis during DNA replication and might also be involved in DNA repair.[4] It is added to the chromatin at the time of DNA replication-associated chromatin assembly. During HSV-1 (herpes simplex virus 1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.[9]

Physical form

Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
5202330605
5202380316
5202321218
5202320614
52023100723

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Jona Merx et al.
Scientific reports, 12(1), 19251-19251 (2022-11-11)
Posttranslational modifications (PTMs) on histone tails regulate eukaryotic gene expression by impacting the chromatin structure and by modulating interactions with other cellular proteins. One such PTM has been identified as serine and threonine glycosylation, the introduction of the ß-N-acetylglucosamine (GlcNAc)
The human histone H3 complement anno 2011.
Ederveen TH
Biochimica et Biophysica Acta, 1809, 577-586 (2011)
Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis.
Tagami H
Cell, 116, 51-61 (2004)
Method of Immunoglobulin Y (IgY) Extraction for Minimally Toxic In Vivo Applications
Nevado Jr, et al.
Philippine journal of Science, 153(1) (2024)
Kami Ahmad et al.
Molecular cell, 9(6), 1191-1200 (2002-06-28)
Two very similar H3 histones-differing at only four amino acid positions-are produced in Drosophila cells. Here we describe a mechanism of chromatin regulation whereby the variant H3.3 is deposited at particular loci, including active rDNA arrays. While the major H3
View All Related Papers

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