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AGRLE-RO

Roche

Agarose LE

low electroendosmosis

Synonym(s):

agarose

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100 G
CN¥2,546.99
500 G
CN¥8,757.71

CN¥2,546.99

Estimated to ship onApril 07, 2025Details

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100 G
CN¥2,546.99
500 G
CN¥8,757.71

About This Item

UNSPSC Code:
12352200

CN¥2,546.99

Estimated to ship onApril 07, 2025Details

biological source

algae (seaweed)

form

powder

packaging

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

manufacturer/tradename

Roche

EEO

0.05 -0.13

transition temp

gel point 36 °C ±1.5

gel strength

>=2,500 g/cm2

foreign activity

Dnase, none detected
Rnase, none detected

shipped in

ambient

storage temp.

20-25°C

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A0576

Agarose

gel strength

>=2,500 g/cm2

gel strength

-

gel strength

≥1200 g/cm2 (1% gel)

gel strength

≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)

EEO

0.05 -0.13

EEO

-

EEO

0.09-0.13

EEO

≤0.12

transition temp

gel point 36 °C ±1.5

transition temp

-

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

foreign activity

Dnase, none detected, Rnase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

biological source

algae (seaweed)

biological source

algae (seaweed)

biological source

algae (Gelidium & Gracilaria)

biological source

-

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.[1]

Application

Agarose LE has been used for:

  • analyzing fragments between 0.2 and 15 kb
  • analysis of PCR products[2]
  • examination of restriction endonucleases
  • digests of plasmid, cosmid, and λ phage DNA
  • electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
  • in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.[3]
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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    Certificates of Analysis (COA)

    Lot/Batch Number
    62212900
    56869900
    56143500
    47210900
    46985000

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    "Impact of Interleukin 16 (IL-16) Gene Polymorphism among

    Seropositive Stages in HIV-1 Infected Patients in North India.
    Kakkar K
    Journal of Virology & Antiviral Research, 8, 006-011 (2016)
    Emily Hodges et al.
    Nature protocols, 4(6), 960-974 (2009-05-30)
    Complementary techniques that deepen information content and minimize reagent costs are required to realize the full potential of massively parallel sequencing. Here, we describe a resequencing approach that directs focus to genomic regions of high interest by combining hybridization-based purification
    Pei Yun Lee et al.
    Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
    Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)

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