Skip to Content
Merck
CN
All Photos(4)

Documents

E9884

Sigma-Aldrich

Ethylenediaminetetraacetic acid

ACS reagent, 99.4-100.6%, powder

Sign Into View Organizational & Contract Pricing

Synonym(s):
(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
EC Number:
MDL number:
UNSPSC Code:
12352106
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.77

Quality Level

grade

ACS reagent

Agency

suitable for SM 4500 - NH3

Assay

99.4-100.6%

form

powder

reaction suitability

reagent type: chelator

impurities

≤0.005% insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

ign. residue

≤0.2%

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

cation traces

Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals: ≤0.001% (byICP-OES)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

Application

Ethylenediaminetetraacetic acid has been used:
  • as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
  • for investigating the effect of papain enzyme on wound debridement and other skin issues
  • for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

CRC Desk Reference of Clinical Pharmacology, 215-215 (1997)
Adsorption of metal-ethylenediaminetetraacetic acid chelates onto lake sediment
Sillanpaa M and Ramo J
Chemosphere, 45, 881-885 (2001)
Henry's Clinical Diagnosis and Management by Laboratory Methods E-book, 28-28 (2011)
Streaming of proteolytic enzyme solutions for wound debridement: a feasibility study
Yaakobi T, et al.
Wounds : a compendium of clinical research and practice, 16, 201-205 (2004)
Renée S Suen et al.
The American journal of pathology, 179(3), 1549-1559 (2011-07-02)
Because both endothelin-1 (ET-1) and angiotensin II (AngII) are independent mediators of arterial remodeling, we sought to determine the role of ET receptor inhibition in AngII-accelerated atherosclerosis and aortic aneurysm formation. We administered saline or AngII and/or bosentan, an endothelin

Articles

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Related Content

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service