A9414
Agarose, low gelling temperature
BioReagent, for molecular biology
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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, LMP agarose, 2-Hydroxyethyl agarose
Recommended Products
biological source
algae (red)
Quality Level
grade
for molecular biology
product line
BioReagent
form
powder
EEO
≤0.10
mp
≤65 °C
transition temp
congealing temperature 26-30 °C
gel strength
≥200 g/cm2 (1% gel)
anion traces
sulfate (SO42-): ≤0.10%
suitability
suitable for electrophoresis
suitable for molecular biology
foreign activity
DNase, RNase, NICKase, none detected
Related Categories
General description
Agarose is a component of agar that is naturally found in the cell wall of red algae. It is hydrophilic and has a high gelling property. It is most commonly employed for gel electrophoresis, to form porous gels to analyze DNA, RNA, or proteins by size. When a voltage is applied to the system the charged nucleic acids migrate through the agarose gel and are separated by size. The stability and gelling properties of agarose make it versitile and it can be used for many other applications in research such as, plaque and comet assays, and even as a scaffolding agent to name a few.
Application
Low-gelling agarose has been used in research:
- for gel electrophoresis, low melting temperature is ideal for DNA extraction from gel -gel scaffold material for tissue engineering using BMSCs
- to embed tissues in 5% agarose for immunohistology
- for preparation of collagen-agarose co-gels to study collagen-matrix interactions in soft tissues
- single-celled gel electrophoresis (comet assay)
- for in-vivo 3-D imaging of zebrafish larvae immobilized on agarose strips
Features and Benefits
- BioReagent suitable for gel electrophoresis
- Band separation range: 200 bp - 25 kB - low gelling temperature is ideal for cell culture and viral plaque assays
- low melting point (LMP) gel is compatible with several DNA recovery methods: phenol/chloroform extractions, recovery columns, electroelution, Beta-Agarase, and freeze/squeeze
- Guaranteed quality tested for use in molecular biology, free of DNase and RNase
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Other Notes
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WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Mechanical and structural contribution of non-fibrillar matrix in uniaxial tension: a collagen-agarose co-gel model
Lake SP and Barocas VH
Annals of Biomedical Engineering, 39(7), 1891-1903 (2011)
Wioletta Skronska-Wasek et al.
American journal of respiratory and critical care medicine, 196(2), 172-185 (2017-03-01)
Chronic obstructive pulmonary disease (COPD), in particular emphysema, is characterized by loss of parenchymal alveolar tissue and impaired tissue repair. Wingless and INT-1 (WNT)/β-catenin signaling is reduced in COPD; however, the mechanisms thereof, specifically the role of the frizzled (FZD)
Spencer P Lake et al.
Journal of biomedical materials research. Part A, 99(4), 507-515 (2011-09-14)
The mechanical properties of soft tissues depend on the collagen fiber network and the surrounding non-fibrillar matrix. The mechanical role of non-fibrillar material remains poorly understood. Our recent study (Lake and Barocas, Ann Biomed Eng 2011) introduced collagen-agarose co-gels as
Insect-like organization of the stomatopod central complex: functional and phylogenetic implications
Thoen HH, et al.
Frontiers in Behavioral Neuroscience, 11(7), 12-12 (2017)
Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastoma multiforme cells
Hinzmann M et al.
International journal of nanomedicine, 15, 2409-2417 (2014)
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