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MMYOMAG-74K

Millipore

MILLIPLEX® Mouse Myokine Magnetic Bead Panel

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples.

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UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 3-40,000 pg/mL
(Osteocrin)
standard curve range: 0.6-9,000 pg/mL
(BDNF)
standard curve range: 128-2,000,000 pg/mL
(Osteonectin (SPARC))
standard curve range: 16-250,000 pg/mL
(Oncostatin M)
standard curve range: 22-350,000 pg/mL
(EPO)
standard curve range: 256-4,000,000 pg/mL
(Myostatin)
standard curve range: 26-400,000 pg/mL
(26Irisin)
standard curve range: 3-40,000 pg/mL
(LIF)
standard curve range: 3-40,000 pg/mL
(Osteocrin/Musclin (OSTN))
standard curve range: 48-750,000 pg/mL
(IL-15)
standard curve range: 5-75,000 pg/mL
(FGF21)
standard curve range: 9-140,000 pg/mL
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

Related Categories

General description

Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various genetically engineered mouse strains and disease models are now serving as exceptional research tools for human myokine study.

MILLIPLEX® Mouse Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO)*, Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Oncostatin M (OSM), Osteocrin/Musclin (OSTN), Osteonectin (SPARC).

*Only available for cells culture samples.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Brain Derived Neurotrophic Factor (BDNF), Erythropoietin (EPO), Fibroblast Growth Factor 21 (FGF-21), Follistatin-like Protein 1 (FSTL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
  • NOTES: BDNF - Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF. OSM - While OSM is barely detectable in the serum/plasma of healthy subjects, a slight increase can be observed in certain disease states. Higher measurements in cell culture samples have also been reported. Erythropoeitin (EPO) is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma.
  • Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
  • Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
  • Research Category: Metabolism
  • Research Subcategory: Obesity, Metabolic Disorders, Inflammation

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Standard curve range: See protocol

Legal Information

6538 is a trademark of American Type Culture Collection
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

WGK

WGK 3

Regulatory Information

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Sofia Garcia et al.
Aging cell, 17(2) (2018-02-11)
PGC-1α is a transcriptional co-activator known as the master regulator of mitochondrial biogenesis. Its control of metabolism has been suggested to exert critical influence in the aging process. We have aged mice overexpressing PGC-1α in skeletal muscle to determine whether
Lars Karlsson et al.
Molecular neurobiology, 58(4), 1465-1481 (2020-11-18)
Physical exercise can improve age-dependent decline in cognition, which in rodent is partly mediated by restoration of an age-dependent decline in neurogenesis. Exercise-inducible myokines in the circulation present a link in muscle-brain crosstalk. The transcription factor PGC-1α regulates the release
Edward O List et al.
Endocrinology, 160(1), 68-80 (2018-11-22)
Global GH receptor-null or knockout (GHRKO) mice have been extensively studied owing to their unique phenotype (dwarf and obese but remarkably insulin sensitive and long-lived). To better understand the influence of adipose tissue (AT) on the GHRKO phenotype, we previously
Susana Peralta et al.
Journal of cell science, 131(13) (2018-06-15)
Mutations in the mitochondrial inner membrane ATPase ATAD3A result in neurological syndromes in humans. In mice, the ubiquitous disruption of Atad3 (also known as Atad3a) was embryonic lethal, but a skeletal muscle-specific conditional knockout (KO) was viable. At birth, ATAD3
Hui J Wang et al.
Nature communications, 11(1), 5099-5099 (2020-10-11)
Mutations in the skeletal muscle Ca2+ release channel, the type 1 ryanodine receptor (RYR1), cause malignant hyperthermia susceptibility (MHS) and a life-threatening sensitivity to heat, which is most severe in children. Mice with an MHS-associated mutation in Ryr1 (Y524S, YS)
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Related Content

Myokine Analysis | MILLIPLEX® Multiplex Assays

Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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