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MAGPMAG-24K

Millipore

MILLIPLEX® Mouse Angiogenesis/Growth Factor Magnetic Bead Panel - Cancer Multiplex Assay

for the simultaneous quantification of multiple analytes

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 90-104%
standard curve range: 1-1,000 pg/mL
(Endothelin-1)
standard curve range: 137-100,000 pg/mL
(Follistatin)
standard curve range: 137-100,000 pg/mL
(HGF)
standard curve range: 137-100,000 pg/mL
(SDF-1)
standard curve range: 14-10,000 pg/mL
(IL-1b)
standard curve range: 14-10,000 pg/mL
(Leptin)
standard curve range: 14-10,000 pg/mL
(MIP-1α)
standard curve range: 14-10,000 pg/mL
(sALK-1)
standard curve range: 14-10,000 pg/mL
(sCD31/ PECAM-1)
standard curve range: 27-20,000 pg/mL
(Angiopoietin-2)
standard curve range: 27-20,000 pg/mL
(EGF)
standard curve range: 27-20,000 pg/mL
(Endoglin)
standard curve range: 27-20,000 pg/mL
(MCP-1)
standard curve range: 27-20,000 pg/mL
(VEGF-C)
standard curve range: 3-2,000 pg/mL
(PLGF-2)
standard curve range: 6-4,000 pg/mL
(TNFα)
standard curve range: 6-4,000 pg/mL
(VEGF-A)
standard curve range: 69-50,000 pg/mL
(FGF-2/FGF-basic)
standard curve range: 69-50,000 pg/mL
(Prolactin)
standard curve range: 69-50,000 pg/mL
(VEGF-D)
standard curve range: 69-50,000 pg/mL
(sFasL)
standard curve range: 7-5,000 pg/mL
(Amphiregulin)
standard curve range: 7-5,000 pg/mL
(Betacellulin)
standard curve range: 7-5,000 pg/mL
(G-CSF)
standard curve range: 7-5,000 pg/mL
(IL-17A)
standard curve range: 7-5,000 pg/mL
(IL-6)
standard curve range: 7-5,000 pg/mL
(KC/CXCL1)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.

MILLIPLEX® Mouse Angiogenesis//Growth Factor Panel, is a 24-plex (for serum/plasma) or 27-plex (for cell culture) kit to be used for the simultaneous quantification of the following analytes: Angiopoietin-2, G-CSF, sFasL, sAlk-1, Amphiregulin, Leptin, IL-1b, Betacellulin, EGF, IL-6, Endoglin, Endothelin-1, FGF-2, Follistatin, HGF, PECAM-1, IL-17, PLGF-2, KC, MCP-1, Prolactin, MIP-1a, SDF-1, VEGF-C, VEGF-D, VEGF-A, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: sALK-1, Amphiregulin, Angiopoietin-2, Betacellulin, sCD31/sPECAM-1, EGF, Endoglin, Endothelin-1, sFasL, FGF-2 (FGF-basic), Follistatin (FST), G-CSF, HGF, IL-1β, IL-6, IL-17A (CTLA8), KC (GRO/CINC-1/CXCL1), Leptin, MCP-1 (CCL2), MIP-1α (CCL3), Placental Growth Factor 2 (PLGF-2), Prolactin, SDF-1 (CXCL12), TNF-α, VEGF-A, VEGF-C, VEGF-D
  • NOTE: Angiopoeitin-2, Betacellulin, and PECAM-1 should not be run in serum/plasma samples
  • Recommended Sample Type: Mouse serum, plasma or tissue/cell culture supernatants or lysates
  • Recommended Sample Dilution: 25 μL per well of diluted serum or plasma; cell/tissue culture samples may require diltion in appropriate control medium
  • Assay Run Time: Overnight (16-20 hours) at 2-8°C
  • Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Sample Type must be selected when configuring this kit. Angiopoeitin-2, Betacellulin, and PECAM-1 should not be run in serum/plasma samples.
Sensitivity: Refer to kit protocol for sensitivities of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Regulatory Information

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Sneha Sundaram et al.
Anticancer research, 39(4), 1729-1738 (2019-04-07)
Monocyte chemotactic protein-1 (MCP1) is a potent adipokine. This study tested the hypothesis that adipose-produced MCP1 contributes to metastasis. In a spontaneous metastasis model of Lewis lung carcinoma (LLC), male adipose MCP1-deficient (Mcp1-/-) and wild-type (WT) mice were fed the
Kenny Schlosser et al.
Thorax, 73(3), 248-261 (2017-09-28)
Elevated plasma levels of angiopoietin-2 (ANGPT2) have been reported in patients with acute lung injury (ALI); however, it remains unclear whether this increase contributes to, or just marks, the underlying vasculopathic inflammation and leak associated with ALI. Here we investigated
Pier-Anne Lachance et al.
PloS one, 8(9), e76078-e76078 (2013-10-03)
During acute inflammation, functioning lymphatics are believed to reduce edema and to provide a transiting route for immune cells, but the extent at which the dermal lymphatic remodeling impacts lymphatic transport or the factors regulating these changes remains unclear. Herein
Aziza R Alrafiah
Biomedicines, 9(9) (2021-09-29)
The present study focused on secondary injury following the middle cerebral artery (MCA) occlusion in rats not linked to the MCA's feeding zone. This entity has been very rarely studied. Additionally, this study investigated the rates of expression of five
Sara Luscieti et al.
Blood, 130(17), 1934-1945 (2017-08-05)
Cellular iron homeostasis is controlled by the iron regulatory proteins (IRPs) 1 and 2 that bind cis-regulatory iron-responsive elements (IRE) on target messenger RNAs (mRNA). We identified profilin 2 (Pfn2) mRNA, which encodes an actin-binding protein involved in endocytosis and
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