MABF2069
Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7
clone 18B7, from mouse
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GXM
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
18B7, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunodepletion: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgG1κ
shipped in
ambient
target post-translational modification
unmodified
General description
Cryptococcus neoformans is an encapsulated yeast-like fungus that is reported to causes systemic infections, including fatal meningoencephalitis. Glucuronoxylomannan (GXM) is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro. The basic structural unit of GXM is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection, and high levels of GXM have been found in serum of patients with cryptococcosis. Clone 18B7 displays significant activity against GXM and is reported act by possibly hydrolyzing the oligosaccharide substrate. This antibody acts as a potent opsonin for Cryptococcus neoforman by increasing its phagocytosis. This antibody may also reduce GXM levels by interfering with the release of polysaccharide from the capsule. Administration of this antibody to mice is shown to rapidly clear serum Cryptococcal antigen. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Martinez, LR., et al. (2004). Infect. Immun. 72(6); 3674 3679; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Devi, SJN et al. (1991). Infect. Immun. 59(10); 3700-3707).
Specificity
Clone 18B7 is a mouse monoclonal antibody that binds to Glucuronoxylomannan (GXM) from Cryptococcus neoformans.
Immunogen
Cryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.
Application
Anti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),
Immunohistochemistry Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunohistochemistry applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46).
Immunodepletion Analysis: A representative lot immunodepleted Glucuronoxylomannan (GXM) in Immunodepletion applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Immunofluorescence Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunofluorescence applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues.
ELISA Analysis: A representative lot detected Glucuronoxylomannan (GXM) in ELISA applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Immunodepletion Analysis: A representative lot immunodepleted Glucuronoxylomannan (GXM) in Immunodepletion applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Immunofluorescence Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunofluorescence applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues.
ELISA Analysis: A representative lot detected Glucuronoxylomannan (GXM) in ELISA applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Quality
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Cells, 11(21) (2022-11-12)
Chimeric antigen receptors (CARs) redirect T cells to recognize a specific target. CAR components play a pivotal role in antigen specificity, structure stability, expression on cell surface, and induction of cellular activation, which together determine the success of CAR T-cell
Nature communications, 13(1), 4074-4074 (2022-07-15)
Cryptococcosis is a potentially lethal disease that is primarily caused by the fungus Cryptococcus neoformans, treatment options for cryptococcosis are limited. Here, we show glucuronoxylomannan, the major polysaccharide component of C. neoformans, induces the recruitment of neutrophilic myeloid-derived suppressor cells
PloS one, 14(8), e0220989-e0220989 (2019-08-10)
Cryptococcus gattii is a capsular fungal pathogen, which causes life-threatening cryptococcosis in immunocompetent individuals. This emerging pathogen is less likely to be recognized by innate immunity compared to traditional Cryptococcus neoformans strains. Previous studies indicate that C-type lectin receptors (CLRs)
Methods in molecular biology (Clifton, N.J.), 2775, 239-255 (2024-05-17)
One of the standard assays for the fungal pathogen Cryptococcus neoformans is the glucuronoxylomannan (GXM) ELISA. This assay utilizes monoclonal antibodies targeted against the critical virulence factor, the polysaccharide (PS) capsule. GXM ELISA is one of the most used assays
European journal of immunology, 51(9), 2281-2295 (2021-03-18)
Cryptococcus gattii is a capsular pathogenic fungus causing life-threatening cryptococcosis. Although the capsular polysaccharides (CPs) of C. gattii are considered as virulence factors, the physiological significance of CP biosynthesis and of CPs themselves is not fully understood, with many conflicting data reported.
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