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ESG1107

Sigma-Aldrich

ESGRO® Recombinant Mouse LIF Protein

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.

Synonym(s):

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF

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200 μG
CN¥7,573.10

CN¥7,573.10

Estimated to ship onApril 21, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1625
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200 μG
CN¥7,573.10

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160801
NACRES:
NA.75

CN¥7,573.10

Estimated to ship onApril 21, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1625
Sign In for Best Price
Request a Bulk Order

Quality Level

100

Assay

>95% (active component mLIF, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

10000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

NCBI accession no.

NM_008501

UniProt accession no.

P09056

shipped in

wet ice

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抗 FAK 抗体，克隆 4.47 clone 4.47, Upstate®, from mouse

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抗 FAK 抗体，克隆 4.47

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16173

Anti-FAK Antibody, clone 4.47, agarose conjugate

Anti-FAK Antibody, clone 4.47, Alexa Fluor™ 488 conjugate clone 4.47, Upstate®, from mouse

16233

Anti-FAK Antibody, clone 4.47, Alexa Fluor™ 488 conjugate

Anti-phospho-FAK (Tyr397) Antibody from rabbit, purified by affinity chromatography

ABT135

Anti-phospho-FAK (Tyr397) Antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

cell based assay: suitable, western blot: suitable

General description

Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Application

Applications for ESGRO Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

At the recommended concentration 107 units of ESGRO is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.

Physical form

ESGRO Supplement is supplied in liquid form as 107 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Format: Purified

Preparation Note

ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.

Storage and Stability

ESGRO Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

Analysis Note

Specific Activity: ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO Supplement, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies(4). 10E7 units is equivalent to approximately 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

    • Certificates of Analysis (COA)

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
    Janebodin, Kajohnkiart, et al.
    Testing, 6, e27526-e27526 (2011)
    Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation.
    Belay, Eyayu, et al.
    Stem Cells, 28, 1760-1771 (2010)
    Xudong Fu et al.
    Science advances, 6(22), eaay5181-eaay5181 (2020-06-12)
    In mouse embryonic stem cell (ESC), a small cell population displays totipotent features by expressing a set of genes that are transiently active in 2-cell-stage embryos. These 2-cell-like (2C-like) cells spontaneously transit back into the pluripotent state. We previously dissected
    Guangdun Peng et al.
    Nature, 572(7770), 528-532 (2019-08-09)
    During post-implantation development of the mouse embryo, descendants of the inner cell mass in the early epiblast transit from the naive to primed pluripotent state1. Concurrently, germ layers are formed and cell lineages are specified, leading to the establishment of
    Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
    Ficz G. et al.
    Nature null
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