ES-006
EmbryoMax® Gelatin Solution
0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Synonym(s):
Porcine Gelatin Coating Solution, porcine gelatin
About This Item
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Product Name
EmbryoMax® 0.1% Gelatin Solution, The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Quality Level
100
200
form
liquid
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
1 of 4
This Item | O4387 | MBD6009 | 11004760001 |
|---|---|---|---|
| form liquid | form solution | form suspension | form - |
| usage 0.1 mL sufficient for 100 RT-PCR reactions | usage 0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20) | usage - | usage sufficient for 50 labeling reactions |
| concentration 50 μM in H2O | concentration 70 μM in H2O | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Application
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
| Wells | _ | Amount/Well |
|---|---|---|
| 96 | 100 μL | |
| 48 | 300 μL | |
| 24 | 0.5 mL | |
| 12 | 1.0 mL | |
| 6 | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
Physical form
Storage and Stability
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Protocols
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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