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1.09202

Millipore

Cefixime Tellurite Supplement

for Escherichia coli, pkg of 10 vials, for the preparation of CT-SMAC Agar

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Synonym(s):
E. coli Selective Supplement, Escherichia coli Selective Supplement, CT supplement
UNSPSC Code:
41106212
NACRES:
NA.74

description

GranuCult™ prime Sorbitol MacConkey (SMAC) Agar (1.00213)

Quality Level

200

form

solid

composition

Cefixime, 0.0025 mg per vial
Potassium tellurite, 1.25 mg per vial

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

2-8°C

suitability

Escherichia coli

General description

CT-Supplement contains cefixime and potassium tellurite. It is used in combination with Sorbitol MacConkey Agar (SMCA) to enhance the isolation of Escherichia coli O157.

Application

CT-Supplement is an additive for the preparation of CT-SMAC Agar for the isolation and differentiation of enterohemorrhagic (EHEC) E. coli 0157:H7-strains from foodstuffs and stool.

Linkage

Replaces: 1092020017

Analysis Note

Appearance
- clearness:
clear
- colour: colourless
pH-value (25 °C): 5.3 - 5.7
Typical composition (per vial): Cefixim 0.025 mg; Potassium Tellurite 1.25 mg.
Growth promotion test in accordance with ISO 11133.
Supplement tested with base Art. 1.09207.
Inoculum on reference medium
- Escherichia coli ATCC 35150 (O157):
10 - 100
- Escherichia coli O157:H7 ATCC 43894: 10 - 100
- Escherichia coli O157:H7 ATCC 43895: 10 - 100
Inoculum
- Escherichia coli ATCC 11775 (WDCM 00090):
>10⁴
- Escherichia coli ATCC 25922 (WDCM 00013): >10⁴
- Staphylococcus aureus ATCC 6538 (WDCM 00032): >10⁴
- Staphylococcus aureus ATCC 25923 (WDCM 00034): >10⁴
Recovery on test medium
- Escherichia coli ATCC 35150 (O157):
≥ 50%
- Escherichia coli O157:H7 ATCC 43894: ≥ 50%
- Escherichia coli O157:H7 ATCC 43895: ≥ 50%
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
partly inhibition
- Escherichia coli ATCC 25922 (WDCM 00013): partly inhibition
- Staphylococcus aureus ATCC 6538 (WDCM 00032): total inhibition
- Staphylococcus aureus ATCC 25923 (WDCM 00034): total inhibition
Colony colour
- Escherichia coli ATCC 35150 (O157):
colourless
- Escherichia coli O157:H7 ATCC 43894: colourless
- Escherichia coli O157:H7 ATCC 43895: colourless
- Escherichia coli ATCC 11775 (WDCM 00090): pink
- Escherichia coli ATCC 25922 (WDCM 00013): pink
Incubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 - H319 - H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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B Tenderis et al.
International journal of food microbiology, 321, 108560-108560 (2020-02-23)
Foodborne illnesses affect the health of consumers worldwide, and thus searching for potential antimicrobial agents against foodborne pathogens is given an increased focus. This research evaluated the influence of sodium lactate (SL), encapsulated (e) and unencapsulated (u) polyphosphates (PP; sodium

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