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1.00188

Millipore

Chromocult® RAMBACH™ Agar

ISO 6579, Specialized media for the isolation and differentiation of Salmonella spp. from food and animal feed, water and other materials.

Synonym(s):

Chromogenic Salmonella Agar, Propylene Glycol Deoxycholate Neutral Red Agar, Salmonella Agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

ISO 6579
suitable for ISO

Quality Level

200

form

powder

technique(s)

microbiological culture: suitable

pH

7.3±0.2 (25 °C)

application(s)

food and beverages

storage temp.

15-25°C

suitability

selective for enterobacteriaceae: Salmonella spp.

Related Categories

General description

Chromocult® RAMBACH™ Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.

Application

The Chromocult® RAMBACH™ Agar can be used for: -
  • The detection of Salmonella in foodstuff, water, animal feed
  • The differential diagnosis of Salmonella species in various samples

Analysis Note

  • The dehydrated medium appears as dark-green color.
  • The prepared medium is opaque and light pink in appearance.
  • The pH value at 25 degrees is in the range of 7.1-7.5.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Rambach is a trademark of Balel S.A.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

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