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Merck
CN

75977

PALCAM Listeria Selective Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

Key Documents

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Product Name

PALCAM Listeria Selective Agar, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.0 g/L
ammonium ferric citrate, 0.5 g/L
esculin, 0.8 g/L
glucose, 0.5 g/L
lithium chloride, 15.0 g/L
D-mannitol, 10.0 g/L
peptone, 23.0 g/L
phenol red, 0.08 g/L
sodium chloride, 5.0 g/L
starch, 1.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
pathogen testing
veterinary
microbiology

suitability

selective and differential for Listeria spp.

Quality Level

200

Related Categories

Application

for differential diagnosis, for the isolation and detection of Listeria monocytogenes from fecal and biological material, food and heavily contaminated environmental specimens.

General description

Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) Listeria Selective Agar is a selective medium. Selectivity is attained mainly by the inhibitory components like lithium chloride, acriflavine, polymyxin and ceftazidime. Two indicator systems confer its diagnostic properties: aesculin and ferric iron and D-mannitol combined with phenol red. This aids in easy differentiation of Listeria spp. from enterococci and staphylococci.

Other Notes

Detection of Listeria species in a range of meat products
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Dissolve 68.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool down to 50°C and add the dissolved contents of 2 vials PALCAM Listeria Selective Supplement (Cat. No. 02336). The completed broth may appear hazy due to starch.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1085
BCCL5434
BCCM5731
BCCK5681
BCCL0748

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Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) agar
Progress in Industrial Microbiology, 395-398 (1995)
C.P.G.L. Gunasinghe et al.
Letters in Applied Microbiology, 18, 156-156 (1994)
Van Nettern, P., et al.
Letters in Applied Microbiology, 12, 20-20 (1991)

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