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1.05878

Millipore

GranuCult® MULLER-KAUFFMANN Tetrathionate Novobiocin broth (base)

according to ISO 6579, selective for Salmonella spp.

Synonym(s):

MKTT Novobiocin Supplement, MKTTn broth, Novobiocin Selective Supplement

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25 G
CN¥1,205.11
100 G
CN¥3,683.57

CN¥1,205.11

Estimated to ship onSeptember 29, 2025Details

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25 G
CN¥1,205.11
100 G
CN¥3,683.57

About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

CN¥1,205.11

Estimated to ship onSeptember 29, 2025Details

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Agency

ISO 11133
according to ISO 6579

Quality Level

200

sterility

non-sterile

form

granular

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

selective for Salmonella spp.

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GF10672938GF52059618GF61030011
Hafnium powder, 45 max. part. size (micron), weight 10 g, purity 95.0+%

GF96885387

Hafnium

Hafnium powder, max. particle size 45 micron, weight 20 g, purity 95.+%

GF10672938

Hafnium

Hafnium lump, 10 mm max. lump size, weight 100 g, purity 97.%

GF52059618

Hafnium

Hafnium lump, 10 mm max. lump size, weight 100 g, purity 97.%

GF61030011

Hafnium

form

powder

form

powder

form

lumps

form

lumps

assay

95.0%

assay

95.0%

assay

97.0%

assay

97%

mp

2227 °C (lit.)

mp

2227 °C (lit.)

mp

2227 °C (lit.)

mp

2227 °C (lit.)

density

13.3 g/cm3 (lit.)

density

13.3 g/cm3 (lit.)

density

13.3 g/cm3 (lit.)

density

13.3 g/cm3 (lit.)

bp

4602 °C (lit.)

bp

4602 °C (lit.)

bp

4602 °C (lit.)

bp

4602 °C (lit.)

General description

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth is a selective medium suitable for the enrichment and isolation of Salmonella. Tetrathionate in the broth suppresses the growth of coliform and other enteric bacteria. It is produced from thiosulfate by adding iodine to the culture medium. Salmonella, Proteus, and some other species of bacteria can reduce tetrathionate. Calcium carbonate buffers the sulphuric acid, which is generated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying bacteria. Brilliant green and novobiocin suppress the growth of primarily Gram-positive bacteria.

Application

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base) is used for the selective enrichment of Salmonella from food, animal feed, and other materials.

Analysis Note

Appearance (clearness): turbid with sediment
Appearance (colour): green
pH-value (25 °C): 7.8 - 8.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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