1.07228
Buffered Peptone Water
GranuCult® prime, ISO 19250, ISO 6579-1, ISO 21528-1, ISO 22964, FDA-BAM, EP, granular, pkg of 0.5-25 kg
Synonym(s):
BPW, Buffered Peptone Solution, GranuCult® BPW, Peptone Water (buffered), Peptone Water, buffered, Peptone Water, phosphate-buffered, Tryptone Phosphate Water
About This Item
Agency
APHA
EP
FDA-BAM
ISO 19250
ISO 21528-1
ISO 22964
ISO 6579-1
ISO 6887
Quality Level
sterility
non-sterile
form
granular
packaging
pkg of 0.5-25 kg
manufacturer/tradename
GranuCult® prime
technique(s)
microbiological culture: suitable
pH
7.2 (37 °C, 25 g/L in H2O, after autoclaving)
solubility
25.5 g/L
bulk density
800 kg/m3
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Cronobacter spp.
Salmonella spp.
enterobacteriaceae
nonselective for bacteria (preliminary enrichment)
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General description
GranuCult® is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Application
Linkage
Storage and Stability
- Store at +15 °C to +25 °C, dry and tightly closed.
- Do not use clumped or discolored medium.
- Protect from UV light (including sunlight).
- For in vitro use only.
- According to EN ISO/FDIS 6579-1, the self-prepared medium can be stored in closed containers at +2 °C to +8 °C in the dark and protected against evaporation for up to six months.
Analysis Note
Appearance (colour): yellowish
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °C
Other Notes
Footnote
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
WGK
WGK 1
Certificates of Analysis (COA)
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Articles
Shigella spp. are predominantly connected to humans and are rarely isolated from other animals, with the exception of primates.
Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions.
Salmonella pass through the entire food chain from animal feed, primary production, and into households or food-service establishments.
Protocols
The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.
The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.
EN ISO 6579-1 standard specifies detection of Salmonella spp. in human consumption, animal feed, and environmental samples.
Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.
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