05-180
Anti-Cortactin (p80/85) Antibody, clone 4F11
clone 4F11, Upstate®, from mouse
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2.5 G
CN¥753.33
5 G
CN¥882.81
25 G
CN¥3,158.37
100 G
CN¥10,143.02
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2.5 G
CN¥753.33
5 G
CN¥882.81
25 G
CN¥3,158.37
100 G
CN¥10,143.02
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
4F11, monoclonal
species reactivity
mouse, bovine, hamster, human, rat, avian
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
1 of 4
This Item | T0557 | C6445 | 30968 |
|---|---|---|---|
| aggregation number 1.9 | aggregation number 6 | aggregation number 2-3 | aggregation number 3-12 |
| description anionic | description anionic | description anionic | description anionic |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| mp 210-215 °C (subl.) (lit.) | mp - | mp - | mp - |
| mol wt micellar avg mol wt 900(lit.) | mol wt micellar avg mol wt 3100 | mol wt micellar avg mol wt 900-1300 | mol wt average mol wt 1200-5000 |
General description
Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding.
Specificity
Recognizes Cortactin.
Immunogen
Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src
Application
Anti-Cortactin (p80/85) Antibody, clone 4F11 is an antibody against Cortactin (p80/85), and has been published and validated for use in IC, IH, IP and WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
routinely evaluated in mouse 3T3/A31 cell lysates or chicken fibroblast src-transformed cell lysate
Target description
80 kDa and 85 kDa
Physical form
Format: Purified
Protein G Purified
Purified immunoglobulin in 10mM PBS, pH 7.4 with 0.05% sodium azide.
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate
Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Direct interaction between caldesmon and cortactin.
Renjian Huang, Gong-Jie Cao, Hongqiu Guo, Jolanta Kordowska, C-L Albert Wang
Archives of Biochemistry and Biophysics null
Xiaolin Sun et al.
Carcinogenesis, 30(12), 2109-2116 (2009-10-22)
Abl interactor 1 (Abi1) is a key regulator of actin polymerization/depolymerization. The involvement of Abi1 in the development of abnormal cytoskeletal functions of cancer cells has recently been reported. It remains unclear, however, how Abi1 exerts its effects in tumor
Inactivation of Src family kinases inhibits angiogenesis in vivo: implications for a mechanism involving organization of the actin cytoskeleton.
Witold W Kilarski, Natalia Jura, Par Gerwins
Experimental Cell Research null
C Dehio et al.
The EMBO journal, 14(11), 2471-2482 (1995-06-01)
Shigella flexneri causes bacillary dysentery in humans by invading epithelial cells of the colon. Cell invasion occurs via bacterium-directed phagocytosis, a process requiring polymerization of actin at the site of bacterial entry. We show that invasion of HeLa cells by
Galpha13 stimulates cell migration through cortactin-interacting protein Hax-1.
Radhika, V; Onesime, D; Ha, JH; Dhanasekaran, N
The Journal of Biological Chemistry null
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