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Key Documents

16-228

Sigma-Aldrich

Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor™ 488

clone 4F11, Upstate®, from mouse

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1 EA
CN¥2,030.19

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Estimated to ship onMay 13, 2025Details

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥2,030.19

Estimated to ship onMay 13, 2025Details

Sign In for Best Price

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4F11, monoclonal

species reactivity

bovine, human, mouse, rat, hamster, avian

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

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Millicoat® Human Collagen Type I Coated Strips (96-Wells)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

NCBI accession no.

NM_002026.2, NM_212474.1, NM_212476.1, NM_212482.1, NM_054034.2, NM_212478.1, NM_212475.1

NCBI accession no.

NM_212482.4

NCBI accession no.

NM_000638.4

NCBI accession no.

NM_000088.3

UniProt accession no.

P02751

UniProt accession no.

P02751

UniProt accession no.

P04004

UniProt accession no.

P02452

species reactivity

human

species reactivity

-

species reactivity

-

species reactivity

human

Specificity

Recognizes Cortactin in rodent and human cells

Immunogen

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Application

Detect Cortactin (p80/85) using this Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor 488 validated for use in FC, IF, WB & IC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Routinely evaluated by immunoblot.

Target description

Mr 80kDa and in avian cells, Mr 80kDa and 85kDa

Physical form

Protein G Purified

Storage and Stability

1 year at 4°C from date of shipment

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, ALEXA FLUOR™ 488-conjugate.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Actions by actin: reciprocal regulation of cortactin activity by tyrosine kinases and F-actin.
    Gunst, Susan J
    The Biochemical Journal, 380, e7-e8 (2004)
    T Katsube et al.
    The Journal of biological chemistry, 273(45), 29672-29677 (1998-10-29)
    Cortactin is an actin filament-binding protein localizing at cortical regions of cells and a prominent substrate for Src family protein-tyrosine kinases in response to multiple extracellular stimuli. Human cortactin has been identified as a protein product of a putative oncogene
    M A McNiven et al.
    The Journal of cell biology, 151(1), 187-198 (2000-10-06)
    The dynamin family of large GTPases has been implicated in the formation of nascent vesicles in both the endocytic and secretory pathways. It is believed that dynamin interacts with a variety of cellular proteins to constrict membranes. The actin cytoskeleton
    Hax-1 is required for Rac1-Cortactin interaction and ovarian carcinoma cell migration.
    Gomathinayagam, R; Muralidharan, J; Ha, JH; Varadarajalu, L; Dhanasekaran, DN
    Genes & Cancer null
    Cortactin phosphorylation as a switch for actin cytoskeletal network and cell dynamics control.
    Lua, Bee Leng and Low, Boon Chuan
    Febs Letters, 579, 577-585 (2005)
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