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1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
purified immunoglobulin, buffered aqueous glycerol solution
Pricing and availability is not currently available.
Pricing and availability is not currently available.
Description
Synonyms
flag antibody, Anti-ddddk, Anti-dykddddk
Anti-ddddk, Anti-dykddddk
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk
Properties
analyte chemical class(es):
analyte chemical class(es):
analyte chemical class(es):
analyte chemical class(es):
proteinsantibody form:
affinity isolated antibodyantibody form:
purified immunoglobulin (Purified IgG1 subclass)antibody form:
purified immunoglobulinantibody form:
purified immunoglobulinbiological source:
mousebiological source:
mousebiological source:
mousebiological source:
capacity:
capacity:
capacity:
capacity:
>0.6 mg/mL, resin binding capacity (FLAG-BAP)clone:
M2, monoclonalclone:
M2, monoclonalclone:
M2, monoclonalclone:
M2, monoclonalconcentration:
1 mg/mLconcentration:
3.8-4.2 mg/mLconcentration:
~1 mg/mLconcentration:
conjugate:
unconjugatedconjugate:
unconjugatedconjugate:
biotin conjugateconjugate:
agarose conjugateshipped in:
wet iceshipped in:
dry iceshipped in:
dry iceshipped in:
wet iceisotype:
IgG1isotype:
IgG1isotype:
IgG1isotype:
IgG1matrix:
matrix:
matrix:
matrix:
(4% agarose bead; 45-165μm bead size)form:
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)form:
buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)form:
buffered aqueous glycerol solutionform:
buffered aqueous glycerol solutionantibody product type:
primary antibodiesantibody product type:
primary antibodiesantibody product type:
primary antibodiesantibody product type:
primary antibodiespurified by:
affinity chromatography (affinity tag purification)purified by:
using Protein Apurified by:
purified by:
immunogen sequence:
DYKDDDDKimmunogen sequence:
DYKDDDDKimmunogen sequence:
DYKDDDDKimmunogen sequence:
species reactivity:
allspecies reactivity:
allspecies reactivity:
allspecies reactivity:
storage temp.:
−20°Cstorage temp.:
−20°Cstorage temp.:
−20°Cstorage temp.:
−20°Ctechnique(s):
technique(s):
western blot: 10 μg/mL (Protein A)technique(s):
dot blot: suitable (chemiluminescent detection)technique(s):
affinity chromatography: suitableimmunoprecipitation (IP): suitable
Usage and Safety
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in:
Browse additional application references in our FLAG® Literature portal.
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunofluorescence
- ELISA
- EIA
- chromatin immunoprecipitation
- electron microscopy
- flow cytometry
- supershift assays
Browse additional application references in our FLAG® Literature portal.
Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.
Learn more product details in our FLAG® application portal.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.
Learn more product details in our FLAG® application portal.
Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Risk and Safety
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 2
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
nwg
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
10 - Combustible liquids
WGK:
WGK 2
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
10 - Combustible liquids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
