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Key Documents

926027

Sigma-Aldrich

NanoFabTX™-DOTAP Lipid Mix

for synthesis of cationic (DOTAP) liposomes

Synonym(s):

Drug delivery, LNP, Microfluidics, NanoFab reagent kit, Nanoformulation, Nanoparticle

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Select a Size

10 X 1 L
¥1,325.92

¥1,325.92

Available to ship on2025年4月16日Details

Request a Bulk Order
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10 X 1 L
¥1,325.92

About This Item

UNSPSC Code:
12162002
NACRES:
NA.23

¥1,325.92

Available to ship on2025年4月16日Details

Request a Bulk Order

Quality Level

100

storage temp.

−20°C

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This Item
M3769M2154M7528
Sigma-Aldrich

M2520

Medium 199

Sigma-Aldrich

M3769

Medium 199

Sigma-Aldrich

M2154

Medium 199

Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

M7528

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

400

General description

NanoFabTx™-DOTAP Lipid Mix includes reagents and optimized protocols with step-by-step instructions for synthesizing cationic liposomes for drug delivery research applications. The modification of liposomes with the cationic lipid, DOTAP, have numerous advantages such as their large-DNA incorporation, high transfection efficiency, and low toxicity. Liposome-based formulations are widely used for drug delivery applications and enable improved therapeutic efficacy of a range of drug types including small molecules, nucleic acids, proteins, and peptides.

Comprehensive protocols for liposome synthesis are included:

  • A lipid film hydration and extrusion protocol.
  • A microfluidics protocol using commercial platforms or syringe pumps.

The microfluidics protocol included with this product uses NanoFabTx™ device kits (911593). These kits come with the microfluidics chips, fittings, and tubing required to get started with microfluidics-based synthesis (compatible microfluidics system or syringe pump required).

Application

NanoFabTx™-DOTAP Lipid Mix is a ready-to-use nanoformulation blend for the synthesis of cationic DOTAP-functionalized liposomes. This kit enables users to encapsulate a wide variety of therapeutic drug molecules for targeted or extended drug delivery without the need for lengthy trial-and-error optimization. NanoFabTx™ kits provide an easy-to-use toolkit for encapsulating a variety of therapeutics in nanoparticles, microparticles, or liposomes. Drug encapsulated particles synthesized with the NanoFabTx™ kits are suitable for biomedical research applications such as oncology, immuno-oncology, gene delivery, and vaccine delivery.

Features and Benefits

  • A ready-to-use nanoformulation blend for the synthesis of cationic liposomes
  • Step-by-step protocols (extrusion or microfluidic) developed and tested by our formulation scientists
  • Flexible synthesis tools to create uniform and reproducible liposomes
  • Optimized to make liposomes around 100 nm with low polydispersity
  • DOTAP surface-functionalized allows for targeting ligand conjugation to enable targeted drug delivery

Legal Information

NANOFABTX is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    MKCS3973
    MKCS3972

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    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

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    Which polymers can make nanoparticulate drug carriers long-circulating?
    Torchilin VP, et al.
    Advanced Drug Delivery Reviews, 16, 141-155 (1995)
    Which polymers can make nanoparticulate drug carriers long-circulating?
    Torchilin V P, et al.
    Advanced Drug Delivery Reviews, 16, 141-155 (1995)
    D Papahadjopoulos et al.
    Proceedings of the National Academy of Sciences of the United States of America, 88(24), 11460-11464 (1991-12-15)
    The results obtained in this study establish that liposome formulations incorporating a synthetic polyethylene glycol-derivatized phospholipid have a pronounced effect on liposome tissue distribution and can produce a large increase in the pharmacological efficacy of encapsulated antitumor drugs. This effect
    T D Madden et al.
    Chemistry and physics of lipids, 53(1), 37-46 (1990-03-01)
    We have shown previously that transmembrane proton gradients can be used to efficiently accumulate biogenic amines [M.B. Bally et al. (1988) Chem. Phys. Lipids 47, 97-107] and doxorubicin [L.D. Mayer, M.B. Bally and P.R. Cullis (1986) Biochim. Biophys. Acta 857
    Yunes Panahi et al.
    Artificial cells, nanomedicine, and biotechnology, 45(4), 788-799 (2017-03-11)
    Liposome is a new nanostructure for the encapsulation and delivery of bioactive agents. There are a lot of bioactive materials that could be incorporated into liposomes including cosmetics, food ingredients, and pharmaceuticals. Liposomes possess particular properties such as biocompatibility, biodegradability;
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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