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Safety Information

SHC016

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

Synonym(s):

MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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This Item
PHR11267617790115
Sulfamethoxazole VETRANAL®, analytical standard

31737

Sulfamethoxazole

Sulfamethoxazole Pharmaceutical Secondary Standard; Certified Reference Material

PHR1126

Sulfamethoxazole

Sulfamethoxazole certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

76177

Sulfamethoxazole

N4-Acetylsulfamethoxazole analytical standard

90115

N4-Acetylsulfamethoxazole

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

Quality Level

100

Quality Level

300

Quality Level

300

Quality Level

100

grade

analytical standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, TraceCERT®

grade

analytical standard

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

product line

VETRANAL®

product line

-

product line

TraceCERT®

product line

-

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
  • in tumor cells for multicolour imaging[1]
  • in human adult low calcium temperature keratinocytes[2]
  • in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)[3]
  • to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation[4]
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
Pricing

SHC001

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert

SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC005

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA, shRNA sequence targeting eGFP

SHC007

MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA, shRNA sequence targeting luciferase

SHC012

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

SHC014

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Zac1 regulates astroglial differentiation of neural stem cells through Socs3
    Schmidt EU, et al.
    Stem Cells, 31(8), 1621-1632 (2013)
    Brain tumour cells interconnect to a functional and resistant network
    Osswald M, et al.
    Nature, 528(7580), 93-93 (2015)
    Sophie Weil et al.
    Neuro-oncology, 19(10), 1316-1326 (2017-04-19)
    Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion
    David M Habiel et al.
    Scientific reports, 7(1), 15444-15444 (2017-11-15)
    Lung fibrosis is an unabated wound healing response characterized by the loss and aberrant function of lung epithelial cells. Herein, we report that extracellular Clusterin promoted epithelial cell apoptosis whereas intracellular Clusterin maintained epithelium viability during lung repair. Unlike normal
    Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
    Avitabile D, et al.
    The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)
    View All Related Papers

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