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Safety Information

SHC014

Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA

Contains a gene encoding TurboGFP, under the control of the UbC promoter

Synonym(s):

MISSION® Control Vectors

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1 MG
CN¥741.91
5 MG
CN¥2,375.11
10 MG
CN¥3,354.73
20 MG
CN¥5,748.88

CN¥741.91

Estimated to ship onApril 21, 2025Details

Request a Bulk OrderRequest more information
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1 MG
CN¥741.91
5 MG
CN¥2,375.11
10 MG
CN¥3,354.73
20 MG
CN¥5,748.88

About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51

CN¥741.91

Estimated to ship onApril 21, 2025Details

Request a Bulk OrderRequest more information

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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Show Differences

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This Item
P49104P49406678848
Piperonylamine 97%

P49503

Piperonylamine

Piperonal 99%

P49104

Piperonal

Piperonyl alcohol 98%

P49406

Piperonyl alcohol

2-Aminomethyl-1,4-benzodioxane 97%

678848

2-Aminomethyl-1,4-benzodioxane

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

density

1.214 g/mL at 25 °C (lit.)

density

-

density

-

density

1.1679 g/mL at 25 °C

refractive index

n20/D 1.564 (lit.)

refractive index

-

refractive index

-

refractive index

n20/D 1.5594

bp

138-139 °C/13 mmHg (lit.)

bp

264 °C (lit.)

bp

-

bp

105-110 °C/3 mmHg

General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Jun Wu et al.
    Cell reports, 33(7), 108395-108395 (2020-11-19)
    The mammalian SWitch/Sucrose Non-Fermentable (SWI/SNF) chromatin-remodeling BAF (BRG1/BRM-associated factor) complex plays an essential role in developmental and pathological processes. We show that the deletion of Baf155, which encodes a subunit of the BAF complex, in the Tie2(+) lineage (Baf155 (CKO)

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