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Safety Information

D5905

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

Synonym(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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500 G
CN¥1,370.57
1 KG
CN¥2,127.82

CN¥1,370.57

Available to ship onApril 07, 2025Details

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500 G
CN¥1,370.57
1 KG
CN¥2,127.82

About This Item

Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
Beilstein:
1212988
EC Number:
231-018-9
MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24893981
NACRES:
NA.83

CN¥1,370.57

Available to ship onApril 07, 2025Details

Request a Bulk Order

Product Name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Show Differences

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XNATD5688XNAT2
Tissue Preparation Solution 30 mL sufficient for 1,000 extractions

T3073

Tissue Preparation Solution

REDExtract-N-Amp™ Tissue PCR Kit sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

XNAT

REDExtract-N-Amp™ Tissue PCR Kit

Dilution Solution Extract-N-Amp Plant Dilution Solution

D5688

Dilution Solution

Extract-N-Amp™ Tissue PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

XNAT2

Extract-N-Amp™ Tissue PCR Kit

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Specificity

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.[1][2]
Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.[3]

Reconstitution

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H319,H341,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
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