True Gel3D™ Hydrogel Troubleshooting Guide

The TrueGel3D™ hydrogel pre-configured kit (TRUE1) is used to prepare a chemically defined hydrogel with stiffness of ~400 Pa (shear modulus) that can be customized to match that of the native cell environment. The TrueGel3D™ hydrogel is formed by crosslinking of RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. The cells are encapsulated during crosslinking and can adhere to the polymer through the RGD peptide and grow within the hydrogel. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala) that enables cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7, and MMP9).

The hydrogel can be further dissolved by TrueGel3D™ Enzymatic Cell Recovery Solution (TRUEENZ) for post-culture analysis including RT-PCR, Western blots and other molecular biological studies.