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安全信息

M3148

Sigma-Aldrich

2-巯基乙醇

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

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别名:
β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇
线性分子式:
HSCH2CH2OH
CAS号:
60-24-2
分子量:
78.13
Beilstein:
773648
EC 号:
200-464-6
MDL编号:
MFCD00004890
UNSPSC代码:
12161504
PubChem化学物质编号:
24896800
NACRES:
NA.31

等级

for molecular biology

质量水平

200

蒸汽密度

2.69 (vs air)

蒸汽压

1 mmHg ( 20 °C)

产品线

BioReagent

检测方案

99% (GC/titration)

形式

liquid

expl. lim.

18 %

反应适用性

reagent type: reductant

浓度

14.3 M (pure liquid)

技术

cell culture | mammalian: suitable
electrophoresis: suitable

折射率

n20/D 1.500 (lit.)

pH值（酸碱度）

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

溶解性

H2O: soluble 1 mL/mL

密度

1.114 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, protease, none detected

储存温度

room temp

SMILES字符串

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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应用

还原剂
β-巯基乙醇或2-巯基乙醇已用于：
  • 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞（鸡B细胞系）的培养
  • 裂解NuLi-1（Normal Lung, University of Iowa-1）细胞
  • 小鼠胚胎干细胞的胰蛋白酶消化
BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键，通常用于 SDS-PAGE 的样品缓冲液，浓度为 5%。 切割分子间（亚基之间）二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。 切割分子内（亚基内）二硫键可使亚基完全变性，从而使每个肽根据其链长迁移，而不受二级结构的影响。

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

靶器官

Liver,Heart

WGK

WGK 3

闪点(°F)

154.4 °F

闪点(°C)

68 °C

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品

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实验方案

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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