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类型
Type VII
表单
buffered aqueous glycerol solution
比活
350-600 units/mg protein
分子量
237 kDa
浓度
2.0-20.0 mg/mL
异质活性
lactic dehydrogenase and creatine phosphokinase ≤0.01%
phosphoglucomutase and myokinase ≤0.05%
储存温度
2-8°C
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此商品 | FLASC | A8937 | A2767 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
一般描述
Research Area: Cell Signaling
Pyruvate kinase plays a role in regulating cell metabolism. There are four pyruvate kinase isoforms in mammals (PKM1, PKM2, PKR, PKL). Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits, arranged in a dimer-of-dimers configuration. Each monomer contains one active site and consists of three main domains- designated A, B, and C-along with a small N-terminal domain. The M2 isoform of pyruvate kinase (PKM2) supports anabolic metabolism and is expressed in cancer and normal tissue.[1]
Pyruvate kinase plays a role in regulating cell metabolism. There are four pyruvate kinase isoforms in mammals (PKM1, PKM2, PKR, PKL). Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits, arranged in a dimer-of-dimers configuration. Each monomer contains one active site and consists of three main domains- designated A, B, and C-along with a small N-terminal domain. The M2 isoform of pyruvate kinase (PKM2) supports anabolic metabolism and is expressed in cancer and normal tissue.[1]
应用
生化/生理作用
丙酮酸激酶催化磷酸基团从磷酸烯醇丙酮酸 (PEP) 转移到 ADP。这种转移产生一分子丙酮酸盐和一分子 ATP。分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
最佳 pH 值：~7.5
最佳 pH 值：~7.5
丙酮酸激酶是一种涉及糖酵解和糖异生的酶。 产品 P7768 来自家兔肌肉，以甘油缓冲水溶液形式提供，已用于 PGM 酶试验，用于测定磷酸甘油酸变位酶活性[6]。 丙酮酸激酶也用于研究丙酮酸激酶（PK）缺乏症。[7]
分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
单位定义
在 37°C、pH 7.6 条件下，1 个单位将每分钟 1.0 μmol 磷酸（烯醇）丙酮酸转化为丙酮酸。
外形
溶于含 0.01 M 磷酸盐的 50% 甘油的溶液，pH 7.0
分析说明
缩二脲法测定蛋白
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding
Anas M, et al.
The Biochemical Journal, 477(18), 3625-3643 (2020)
James F Zawada
Methods in molecular biology (Clifton, N.J.), 805, 31-41 (2011-11-19)
Crude cell-free extracts are useful tools for investigating biochemical phenomena and exploiting complex enzymatic processes such as protein synthesis. Extracts derived from E. coli have been used for over 50 years to study the mechanism of protein synthesis. In addition
Yinhua Zhang et al.
The Journal of biological chemistry, 279(35), 37185-37190 (2004-07-06)
Phosphoglycerate mutases catalyze the interconversion of 2- and 3-phosphoglycerate in the glycolytic and gluconeogenic pathways. They exist in two unrelated forms that are either cofactor (2,3-diphosphoglycerate)-dependent or cofactor-independent. The two enzymes have no similarity in amino acid sequence, tertiary structure
I Mor et al.
Oncogene, 31(6), 683-693 (2011-07-05)
Death-associated protein kinase (DAPk), a multi-domain serine/threonine kinase, regulates numerous cell death mechanisms and harbors tumor suppressor functions. In this study, we report that DAPk directly binds and functionally activates pyruvate kinase M2 (PKM2), a key glycolytic enzyme, which contributes
Studies on pyruvate kinase (PK) deficiency. II. Electrophoretic, kinetic and immunological studies on pyruvate kinase of erythrocytes and other tissues.
K Imamura et al.
Journal of biochemistry, 74(6), 1165-1175 (1973-12-01)
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