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安全信息

ND7/23 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090903, Mouse x Rat Hybridoma nerve, Neuronal

别名:

ND7/23 Cells

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About This Item

UNSPSC代码:
41106514

生物来源

Mouse x Rat Hybridoma nerve

包装

tube of 5 μg 92090903-DNA-5UG
pkg of vial of cells 92090903-1VL

生长模式

Adherent

核型

Not specified

形态学

Neuronal

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

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species reactivity

human, mouse

species reactivity

human, Xenopus

species reactivity

human, zebrafish, mouse, Xenopus, chicken, fish

species reactivity

human

clone

monoclonal

clone

monoclonal

clone

monoclonal

clone

1.2.1.5.4, monoclonal

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ChIP: suitable, ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, multiplexing: suitable, western blot: suitable

Gene Information

human ... TCF7L2(6934)

Gene Information

-

Gene Information

human ... TBP(6908)

Gene Information

human ... CTCF(10664)

细胞系来源

Mouse neuroblastoma x Rat neurone hybrid

细胞系描述

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

应用

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.
Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

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