跳转至内容
Merck
CN

CASP3F

Sigma-Aldrich

胱天蛋白酶3检测试剂盒,荧光法

别名:

胱天蛋白酶3(Caspase-3)活性检测试剂盒(荧光法)

登录查看公司和协议定价


About This Item

EC 号:
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

质量水平

运输

dry ice

储存温度

−20°C

基因信息

human ... CASP3(836)

一般描述

胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC),发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线,计算酶解产物AMC浓度(AMC标准品已包含在试剂盒中)。

应用

胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。

单位定义

pH 7.4、25 °C下,每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。

仅试剂盒组分

产品编号
说明

  • Water (17-18 megaOhm) 125 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • A1086Ac-DEVD-AMC substrate 2.5 mg化学品安全说明书

  • A0835Ac-DEVD-CHO inhibitor .5 mg化学品安全说明书

  • A98917-Amino-4-methylcoumarin standard 1 mg化学品安全说明书

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin
Kaestner,L et al.
Photochemistry and Photobiology, 2, 660-667 (2003)
Alfia Khaibullina et al.
Blood cells, molecules & diseases, 86, 102493-102493 (2020-09-15)
Strokes are feared complications of sickle cell disease (SCD) and yield significant neurologic and neurocognitive deficits. However, even without detectable strokes, SCD patients have significant neurocognitive deficits in domains of learning and memory, processing speed and executive function. In these
Irshad Ali et al.
The Annals of thoracic surgery, 93(1), 282-288 (2011-11-26)
We previously reported that the cytochrome P450 product 20-hydroxyeicosatetraenoic acid has prosurvival effects in pulmonary artery endothelial cells and ex vivo pulmonary arteries. We tested the potential of a 20-hydroxyeicosatetraenoic acid analog N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine (20-5,14-HEDGE) to protect against lung ischemic reperfusion
N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
Sau Wai Hung et al.
PloS one, 8(8), e71196-e71196 (2013-08-14)
We evaluated the potential of an investigational histone methylation reversal agent, 3-deazaneplanocin A (DZNep), in improving the chemosensitivity of pancreatic cancer to nucleoside analogs (i.e., gemcitabine). DZNep brought delayed but selective cytotoxicity to pancreatic cancer cells without affecting normal human

商品

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门