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用途
sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)
质量水平
运输
dry ice
储存温度
−20°C
基因信息
human ... CASP3(836)
一般描述
胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC)，发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线，计算酶解产物AMC浓度（AMC标准品已包含在试剂盒中）。
应用
胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。
单位定义
pH 7.4、25 °C下，每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。
仅试剂盒组分
产品编号
说明
- Water (17-18 megaOhm) 125 mL
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
V A Smith et al.
The British journal of ophthalmology, 88(5), 619-625 (2004-04-20)
Doxycycline is a broad spectrum antibiotic that chelates metal ions and is frequently used as part of the treatment of ocular surface diseases. Its therapeutic value has been ascribed to an ability to inhibit matrix metalloproteinase (MMP) activity and both
N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
Sau Wai Hung et al.
PloS one, 8(8), e71196-e71196 (2013-08-14)
We evaluated the potential of an investigational histone methylation reversal agent, 3-deazaneplanocin A (DZNep), in improving the chemosensitivity of pancreatic cancer to nucleoside analogs (i.e., gemcitabine). DZNep brought delayed but selective cytotoxicity to pancreatic cancer cells without affecting normal human
Irshad Ali et al.
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We previously reported that the cytochrome P450 product 20-hydroxyeicosatetraenoic acid has prosurvival effects in pulmonary artery endothelial cells and ex vivo pulmonary arteries. We tested the potential of a 20-hydroxyeicosatetraenoic acid analog N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine (20-5,14-HEDGE) to protect against lung ischemic reperfusion
商品
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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