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Merck
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CASP3F

胱天蛋白酶3检测试剂盒,荧光法

别名:

胱天蛋白酶3(Caspase-3)活性检测试剂盒(荧光法)

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关于此项目

UNSPSC Code:
12161503
EC Number:
247-454-8
NACRES:
NA.84
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usage

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

shipped in

dry ice

storage temp.

−20°C

Quality Level

Gene Information

human ... CASP3(836)

Application

胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。

General description

胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC),发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线,计算酶解产物AMC浓度(AMC标准品已包含在试剂盒中)。

Other Notes

pH 7.4、25 °C下,每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

常规特殊物品
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