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用途
sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)
质量水平
运输
dry ice
储存温度
−20°C
基因信息
human ... CASP3(836)
一般描述
胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC)，发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线，计算酶解产物AMC浓度（AMC标准品已包含在试剂盒中）。
应用
胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。
单位定义
pH 7.4、25 °C下，每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。
仅试剂盒组分
产品编号
说明
- Water (17-18 megaOhm) 125 mL
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
WGK
WGK 3
法规信息
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商品
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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