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推荐产品
产品名称
BL21(DE3)电感受态细胞, for protein expression
生物来源
Escherichia coli
等级
Molecular Biology
for molecular biology
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
运输
dry ice
储存温度
−70°C
1 of 4
此商品 | CMC0024 | CMC0014 | CMC0011 |
|---|---|---|---|
| growth mode adherent or suspension | growth mode adherent or suspension | growth mode adherent or suspension | growth mode adherent or suspension |
| biological source Escherichia coli | biological source Escherichia coli | biological source Escherichia coli | biological source Escherichia coli |
| morphology rod shaped | morphology rod shaped | morphology rod shaped | morphology rod shaped |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
一般描述
BL21（DE3）电转感受态细胞是第一个在相同细胞中实现高效克隆和高水平蛋白质表达的细胞。
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
特点和优势
BL21（DE3）电转感受态细胞（>5 × 109 cfu/μg）前所未有的转化效率消除了将质粒从克隆菌株转移到表达菌株的需要，从而为典型的克隆和表达实验节省了数天的工作时间。
组分
- BL21（DE3）电转感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
法规信息
新产品
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实验方案
Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.
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