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Merck
CN

CC076

人IV型胶原蛋白

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

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关于此项目

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
liquid
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Mol wt:
300 kDa
Assay:
95% (Human collagen type IV, SDS-PAGE)

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产品名称

人IV型胶原蛋白, 1 mg/mL, solution, BioReagent, suitable for cell culture

biological source

human

assay

95% (Human collagen type IV, SDS-PAGE)

form

liquid
solution

mol wt

300 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<5% Human collagen types I-III, V, VI, and non-collagen proteins

input

sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

solubility

water: soluble at 20 °C

NCBI accession no.

NM_001845.4

UniProt accession no.

P02462

binding specificity

Peptide Source: Laminin

Peptide Source: Nidogen

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... COL4A1(1282)

相关类别

Analysis Note

通过形成微纤维的能力证实了天然结构的保留。

Application

胶原蛋白IV（来自人胎盘）适合用作细胞培养研究的包被材料和胶原凝胶形成。 人胶原蛋白IV已用于：制备侵袭试验用胶原凝胶，在细胞培养系统中用作支持基质，以评估单核细胞的侵袭能力作为胞外基质(ECM)蛋白包被硝酸纤维素膜芯片片基用于芯片分析包被P96-O2板的微通道，为共培养系统中的内皮细胞(hMVEC)和肾近曲小管上皮细胞(hRPTEC)提供贴附和生长表面

Biochem/physiol Actions

人胶原蛋白IV与Alport和Goodpasture综合征有关，也与严重的风湿性疾病和皮肤病有关，如获得性大疱性表皮松解症。

Disclaimer

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

General description

人IV型胶原是皮肤基底膜的主要组成成分。具有天然的三螺旋结构，是由2条α1-样和1条α2-样基因组成的异源三聚体蛋白。4型胶原在多细胞组织中形成折叠状胞外基质，包围特定类型的细胞，比如脂肪细胞、骨骼肌细胞、心肌细胞和平滑肌细胞。 经过胃蛋白酶温和处理后，将人胶原蛋白IV从洗涤过的解剖组织中提取到稀乙酸中。通过差异盐沉淀法纯化4型胶原。纯度和天然螺旋结构的保留通过SDS-PAGE和与抗特定类型胶原单克隆抗体的反应来控制。分子量：300kDa；溶解度：10mg/mL 来源：人胎盘，HBsAg和HIV抗体阴性

Physical form

液体纯化蛋白。 在100μ L的0.5 M乙酸溶液中，pH 2.5。 无盐，无防腐剂。

Preparation Note

可在-20ºC保存长达12个月。 应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Collagen IV in Normal Skin and in Pathological Processes
Abreu-Velez AM, et al.
Journal of Separation Science, 4(1) (2012)
Steady-state monitoring of oxygen in a high-throughput organ-on-chip platform enables rapid and non-invasive assessment of drug-induced nephrotoxicity
Kann SH, et al.
Journal of Separation Science, 148(14), 3204-3216 (2023)
Ana Maria Abreu-Velez et al.
North American journal of medical sciences, 4(1), 1-8 (2012-03-07)
Type IV collagen is a type of collagen found primarily in the skin within the basement membrane zone. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers are thus link head-to-head
Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain.
Bentz, H, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 3168-3172 (1983)
5.16 - Collagen Formation and Structure
Mander L and Liu HW
Comprehensive Natural Products II: Chemistry and Biology (2010)
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