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Merck
CN

CC076

Human Collagen Type IV

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Key Documents

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Product Name

Human Collagen Type IV, 1 mg/mL, solution, BioReagent, suitable for cell culture

biological source

human

assay

95% (Human collagen type IV, SDS-PAGE)

form

liquid
solution

mol wt

300 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<5% Human collagen types I-III, V, VI, and non-collagen proteins

input

sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

solubility

water: soluble at 20 °C

NCBI accession no.

NM_001845.4

UniProt accession no.

P02462

binding specificity

Peptide Source: Laminin

Peptide Source: Nidogen

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... COL4A1(1282)

Related Categories

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Application

Collagen IV from the human placenta is suitable for use as a coating material for cell culture studies and the formation of collagen gels. Human Collagen Type IV has been used: to prepare collagen gel for the invasion assay to provide a supportive matrix for assessing the invasive capacity of monocytes in a cell culture systemas an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slidesto coat the microchannels of the P96-O2 plate, and provide a surface for the adhesion and growth of endothelial cells (hMVECs) and renal proximal tubular epithelial cells (hRPTECs) in a co-culture system

Biochem/physiol Actions

Human collagen type IV is associated with Alport and Goodpasture′s syndrome, as well as several rheumatological and dermatological diseases including, acquired epidermolysis bullosa.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Human collagen type IV is a major structural element predominantly found in the basement membrane of the skin. It is a native triple helix and heterotrimeric protein containing two α1-like and one α2-like genes. Type 4 collagen forms a sheet-like extracellular matrix in multicellular tissues that exists around certain cell types like adipocytes, skeletal muscle cells, heart muscle cells, and smooth muscle cells. Human Collagen IV is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type 4 was purified by using differential salt precipitation. Purity and retention of the native helical structure were controlled by SDS-PAGE and by reaction with anti-collagen type-specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL Product Source: Human placenta, negative for HBsAg and HIV antibodies

Physical form

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Preparation Note

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neutrophil Extracellular Traps Induce Tissue-Invasive Monocytes in Granulomatosis With Polyangiitis
Akiyama M, et al.
Frontiers in Immunology, 10, 2617-2617 (2019)
Samuel H Kann et al.
The Analyst, 148(14), 3204-3216 (2023-06-12)
High-throughput, rapid and non-invasive readouts of tissue health in microfluidic kidney co-culture models would expand their capabilities for pre-clinical assessment of drug-induced nephrotoxicity. Here, we demonstrate a technique for monitoring steady state oxygen levels in PREDICT96-O2, a high-throughput organ-on-chip platform
5.16 - Collagen Formation and Structure
Mander, et al.
Comprehensive Natural Products II: Chemistry and Biology, 1 (2010)
Mitsuhiro Akiyama et al.
Frontiers in immunology, 10, 2617-2617 (2019-12-05)
Objective: Granulomatosis with polyangiitis (GPA) is a multi-organ vasculitic syndrome typically associated with neutrophil extracellular trap (NET) formation and aggressive tissue inflammation. Manifestations in head and neck (H&N) GPA include septal perforations, saddle-nose deformities, bony erosions of the orbital and
5.16 - Collagen Formation and Structure
Mander L and Liu HW
Comprehensive Natural Products II: Chemistry and Biology (2010)
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Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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