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E8408

Sigma-Aldrich

pFLAG-CTC™ Expression Vector

Bacterial vector for cytoplasmic expression of C-terminal FLAG fusion proteins

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0.5 ML
CN¥2,240.04
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CN¥4,144.31

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0.5 ML
CN¥2,240.04
1 ML
CN¥4,144.31

About This Item

UNSPSC代码:
12352200

CN¥2,240.04

请联系客服了解存货情况
新价格，新优惠！

标签

FLAG® tagged

等级

Molecular Biology

表单

buffered aqueous solution

运输

dry ice

储存温度

−20°C

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assay range

accuracy: 84-108%, standard curve range: 21-15,000 pg/mL
(sRAGE), standard curve range: 3-2,500 pg/mL
(Amyloid β40), standard curve range: 27-20,000 pg/mL
(Amyloid β42), standard curve range: 14-10,000 pg/mL
(S100B), standard curve range: 7-5,000 pg/mL
(GDNF)

assay range

accuracy: 107%
(DJ1), accuracy: 108%
(a-Synuclein), accuracy: 123%
(TG2), standard curve range: 12-50,000 pg/mL
(GFAP), standard curve range: 15-60,000 pg/mL
(NSE), standard curve range: 20-80,000 pg/mL
(UCHL1), standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein), standard curve range: 61-250,000 pg/mL
(DJ1), standard curve range: 61-250,000 pg/mL
(TG2)

assay range

accuracy: 69-92%
(Cerebrospinal fluid (CSF)), accuracy: 71-107%
(Serum), standard curve range: 0.03-25 ng/mL
(SOD2), standard curve range: 0.04-30 ng/mL
(Kallikrein-6), standard curve range: 0.04-30 ng/mL
(SOD1), standard curve range: 0.08-60 ng/mL
(Contactin-1), standard curve range: 0.14-100 ng/mL
(Osteopontin (OPN)), standard curve range: 1.37-1,000 ng/mL
(AGT), standard curve range: 4.12-3,000 ng/mL
(Fetuin A)

assay range

accuracy: 108%
(RANTES), accuracy: 108%
(sICAM-1), accuracy: 111%
(Cathepsin D), accuracy: 123%
(PDGF-AA), accuracy: 137%
(PDGF-AB/BB), standard curve range: 2-10,000 pg/mL
(RANTES), standard curve range: 2.4-10,000 pg/mL
(BDNF), standard curve range: 2.4-10,000 pg/mL
(PAI-1 (total)), standard curve range: 2.4-10,000 pg/mL
(PDGF-AA), standard curve range: 24-100,000 pg/mL
(Cathepsin D), standard curve range: 24-100,000 pg/mL
(MPO), standard curve range: 24-100,000 pg/mL
(NCAM), standard curve range: 24-100,000 pg/mL
(PDGF-AB/BB), standard curve range: 24-100,000 pg/mL
(sICAM-1), standard curve range: 61-250,000 pg/mL
(sVCAM-1)

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

一般描述

The pFLAG-CTC™ Expression Vector is a 5.3 kb E. coli expression vector used for cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein. The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products.

C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb vE. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.

Vector Maps and Sequences

组分

  • pFLAG-CTC™ Expression Vector 10 μg (E5394) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pFLAG-CTS™-BAP Control Plasmid 1 μg (P7707) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

原理

The promoter-regulatory region of the strong tac promoter (a hybrid of the trp and lac promoters from E.coli) drives transcription of ORF-FLAG fusion constructs. Control of transcription is regulated by the presence of the lacO sequences and inclusion of the lac repressor gene (lacI) on the plasmid.

法律信息

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CTC is a trademark of Sigma-Aldrich Co. LLC
pFLAG-CTS is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

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    K A Mookhtiar et al.
    Diabetes, 45(12), 1670-1677 (1996-12-01)
    Glucokinase is a critical component of the physiological glucose sensor found in cell types that are responsive to changes in plasma glucose levels. The acute regulation of glucokinase activity has been shown to occur via a regulatory protein found in
    Chlamydia trachomatis Secretion of an Immunodominant Hypothetical Protein (CT795) into Host Cell Cytoplasm
    Manli Qi, Lei Lei, Siqi Gong, Quanzhong Liu, et al
    Journal of Bacteriology, 193, 24698-22509 (2011)
    Veronika Kucharova et al.
    PloS one, 8(6), e66429-e66429 (2013-07-11)
    mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability.
    Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas.
    Robin Couch et al.
    BioTechniques, 32(6), 1230-1230 (2002-06-21)
    Ram P Garg et al.
    Microbiology (Reading, England), 156(Pt 2), 472-483 (2009-11-07)
    Streptomyces antibiotic regulatory proteins (SARPs) have been shown to activate transcription by binding to a tandemly arrayed set of heptameric direct repeats located around the -35 region of their cognate promoters. Experimental evidence is presented here showing that vlmI is
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