Mammalian Genomic DNA Miniprep Kit

For purification of genomic DNA from a variety of mammalian sources

Description: The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources (Figure 1 and Figure 2). These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format (G1N10, G1N70, G1N350).

Procedure

Mammalian cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. DNA is bound to the spin column membrane and the remaining lysate is removed by centrifugation. A filtration column is used to remove cell debris. After washing to remove contaminants, the DNA is eluted with buffer into a collection tube. The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion, ligation, and PCR.

Features and Benefits

• Typical DNA yields of 25 µg from 2 x 106 cultured cells or 30 µg from 25 mg of tissue (Figure 4)
• Preparation time is only 20 minutes after lysis
• Purified genomic DNA has A260/A280 ratios between 1.6 and 1.9
• No need for mechanical homogenization
• 40% more purification preps offered than other leading supplier

Storage

Room Temperature
R: 20/21/22 S: 26-36

Application

Purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion (Figure 3), ligation, and PCR (Figure 4).