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G1N70

Sigma-Aldrich

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 70 purifications

Synonym(s):

Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 70 purifications

Quality Level

200

technique(s)

DNA purification: suitable

storage temp.

15-25°C

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General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

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Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%

P2308

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications

G1N10

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 10 purifications

R6148

RNase A solution

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Human herpesvirus (HHV) 8, an essential etiologic agent of Kaposi sarcoma, is also associated with several lymphoproliferative disorders. The involvement of HHV 8 in mycosis fungoides (MF) and large plaque parapsoriasis (LPP) is controversial, with contradictory reports from various countries
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