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G1N10

Sigma-Aldrich

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Synonym(s):

Mammalian Genomic DNA Miniprep, Gen Elute

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100 TESTS
¥13,220.39

¥13,220.39

Estimated to ship on2025年8月05日Details

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100 TESTS
¥13,220.39

About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

¥13,220.39

Estimated to ship on2025年8月05日Details

usage

sufficient for 10 purifications

Quality Level

200

storage temp.

15-25°C

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manufacturer/tradename

Assurance GDS®, BioControl Systems 71018-100

manufacturer/tradename

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manufacturer/tradename

Assurance GDS®, BioControl Systems 71005-100

manufacturer/tradename

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suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

suitability

Escherichia coli (O157:H7)

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

compatibility

for use with GDS Rotor-Gene

compatibility

for use with GDS Rotor-Gene

compatibility

for use with GDS Rotor-Gene

compatibility

for use with GDS Rotor-Gene

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg proteinSDS
  • R6148RNase A solutionSDS

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P2308

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E1510

Ethidium bromide solution, BioReagent, Molecular Biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, Molecular Biology, low EEO

G1N70

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

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Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications

R6148

RNase A solution

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Regulatory Information

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    SLCB3846

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    Mammalian Genomic DNA Miniprep Kit

    GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service