LC/MS Analysis of Amino Acids on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 13 mM ammonium acetate in 10:90 (v/v) water: acetonitrile; [B] 13 mM ammonium acetate in water

gradient

held at 0% B for 1 min; 0 to 90% B in 19 min

flow rate

1.0 mL/min

column temp.

35 °C

detector

ESI(+)

injection

10 μL

sample

10 mg/L in Mobile Phase A