HPLC Analysis of Spice Cannabinoids on Ascentis® Express F5
Materials
analytical column
standard
Spice Cannabinoid Mix solution
100 μg/mL each component in acetonitrile, ampule of 1.0 mL, certified reference material, Cerilliant®HU-210 solution
100 μg/mL in methanol, (Spice Cannabinoid), ampule of 1 mL, certified reference material, Cerilliant®JWH-018 solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-073 solution
100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®JWH-073 N-(3-hydroxybutyl) metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase
[A] 50 mM ammonium formate; [B] water; [C] acetonitrile; (10:35:55, A:B:C)
flow rate
0.6 mL/min
pressure
4075 psi (281 bar)
column temp.
30 °C
detector
UV, 200 nm
injection
3 μL
sample
100 μg/mL in 45:55 water:acetonitrile
Description
Analysis Note
Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provide a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of eight of these compounds on Ascentis Express F5 column is shown here. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany