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HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V

HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V application for HPLC

Materials

analytical column

Product No.
Description
Pricing

11024AST

Astec® CHIROBIOTIC® V Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

S9944

Sibutramine hydrochloride monohydrate

≥98% (HPLC), solid

CONDITIONS

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

15 mM ammonium formate in methanol

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

1 mg/mL in methanol

Description

Analysis Note

Sibutramine, one of a few drugs approved for long-term treatment of obesity, is a chiral compound. The (R) enantiomer has the desired effect of reducing body weight and food intake, but the (S) enantiomer has the opposite effect: A perfect example of why chiral HPLC is important to pharmaceutical and clinical analysts. Astec® CHIROBIOTIC® V successfully separates sibutramine enantiomers, using a simple, MS-friendly mobile phase.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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