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HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 15 cm × 7.8 mm, 3 μm particle size, stainless steel

standard

Product No.
Description
Pricing

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Zenix SEC-300, 15 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777030)

mobile phase

150 mM sodium phosphate, pH 7

flow rate

as indicated on figure

column temp.

ambient

detector

UV, 214 nm

injection

10 μL

sample

each 1 mg/mL except uracil, 2.5 mg/mL

Description

Legal Information

Zenix is a registered trademark of Sepax Technologies