HPLC Analysis of Proteins by Size Exclusion on Zenix^® Columns, Effect of Pore Size

column

Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm, 150 Å particles (Z777018)

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777033)

mobile phase

150 mM sodium phosphate, pH 7

flow rate

1 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

10 μL

sample

each 1 mg/mL except uracil, 2.5 mg/mL