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HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C)

HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

12024AST

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

guard cartridge

Product No.
Description
Pricing

12100AST

Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

standard

Product No.
Description
Pricing

H6034

L-Histidine

suitable for cell culture, meets EP, USP testing specifications, from non-animal source

H8000

L-Histidine

ReagentPlus®, ≥99% (TLC)

H7750

DL-Histidine

≥99% (TLC)

H3751

D-Histidine

≥98% (TLC)

H3911

L-Histidine

53319

L-Histidine

BioUltra, ≥99.5% (NT)

588644

DL-Histidine-1-13C

99 atom % 13C, 98% (CP)

73767

L-Histidine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.006, A:B:C)

flow rate

0.5 mL/min

column temp.

15 °C

detector

UV, 205 nm

injection

10 μL

sample

200 μg /mL in 50:50 water:methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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