Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations.

Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations.

Parasites & vectors (2015-05-30)

Marcelo Ramalho-Ortigão, Iliano V Coutinho-Abreu, Valdir Q Balbino, Carlos Alberto S Figueiredo, Rami Mukbel, Hussan Dayem, Hanafi A Hanafi, Shabaan S El-Hossary, Emad El-Din Y Fawaz, Mahmoud Abo-Shehada, David F Hoel, Gwen Stayback, Mariha Wadsworth, Douglas A Shoue, Jenica Abrudan, Neil F Lobo, Andrew R Mahon, Scott J Emrich, Shaden Kamhawi, Frank H Collins, Mary Ann McDowell

PMID26022221

ABSTRACT

The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. The analysis of PpSP15 expression from field populations revealed significant intra- and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections. Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.

MATERIALS

Product Number

Brand

Product Description

718483

Sigma-Aldrich

Silica, nanopowder, 99.8% trace metals basis

637246

Sigma-Aldrich

Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis

420794

Sigma-Aldrich

LUDOX^® SM colloidal silica, 30 wt. % suspension in H₂O

420808

Sigma-Aldrich

LUDOX^® LS colloidal silica, 30 wt. % suspension in H₂O

420883

Sigma-Aldrich

LUDOX^® CL colloidal silica, 30 wt. % suspension in H₂O

381276

Supelco

Silica, 99.8%

440345

Supelco

Glass spheres

S5505

Sigma-Aldrich

Silica, fumed, powder, 0.2-0.3 μm avg. part. size (aggregate)

S5130

Sigma-Aldrich

Silica, fumed, powder

N7000

Sigma-Aldrich

1-Naphthyl phosphate monosodium salt monohydrate, ≥98% (titration), powder

777242

Sigma-Aldrich

Silica, mesostructured, SBA-15, 99% trace metals basis

748161

Sigma-Aldrich

Silica, nanoparticles, mesoporous, 200 nm particle size, pore size 4 nm

560979

Sigma-Aldrich

Silica, mesostructured, MSU-F (cellular foam)

421553

Sigma-Aldrich

Silica

634867

Sigma-Aldrich

Silicon dioxide, single crystal substrate, optical grade, 99.99% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

420824

Sigma-Aldrich

LUDOX^® HS-30 colloidal silica, 30 wt. % suspension in H₂O

342890

Sigma-Aldrich

Silicon dioxide, −325 mesh, 99.5% trace metals basis

420786

Sigma-Aldrich

LUDOX^® TM-40 colloidal silica, 40 wt. % suspension in H₂O

643645

Sigma-Aldrich

Silica, mesostructured, MCM-41 type (hexagonal)

637238

Sigma-Aldrich

Silicon dioxide, nanopowder, 10-20 nm particle size (BET), 99.5% trace metals basis

85356

Sigma-Aldrich

Silicon dioxide, granular, ≥99.9%

420840

Sigma-Aldrich

LUDOX^® AS-40 colloidal silica, 40 wt. % suspension in H₂O

420859

Sigma-Aldrich

LUDOX^® TMA colloidal silica, 34 wt. % suspension in H₂O

204358

Sigma-Aldrich

Sand, white quartz, ≥99.995% trace metals basis

420891

Sigma-Aldrich

LUDOX^® CL-X colloidal silica, 45 wt. % suspension in H₂O

420832

Sigma-Aldrich

LUDOX^® AS-30 colloidal silica, 30 wt. % suspension in H₂O

420778

Sigma-Aldrich

LUDOX^® TM-50 colloidal silica, 50 wt. % suspension in H₂O

420875

Sigma-Aldrich

LUDOX^® AM colloidal silica, 30 wt. % suspension in H₂O

420816

Sigma-Aldrich

LUDOX^® HS-40 colloidal silica, 40 wt. % suspension in H₂O

C9157

Sigma-Aldrich

Activated charcoal, suitable for cell culture, suitable for plant cell culture