Skip to Content
Merck
CN
  • Artificial receptors for the extraction of nucleoside metabolite 7-methylguanosine from aqueous media made by molecular imprinting.

Artificial receptors for the extraction of nucleoside metabolite 7-methylguanosine from aqueous media made by molecular imprinting.

Journal of chromatography. A (2014-09-15)
Aleksandra Krstulja, Coralie De Schutter, Patrick Favetta, Panagiotis Manesiotis, Luigi A Agrofoglio
ABSTRACT

A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using (1)H NMR titrations whereby methacrylic acid was shown to be the strongest receptor with and affinity constant of 621±51Lmol(-1)vs. 110±16Lmol(-1) for acrylamide. The best performing polymers were prepared using methanol as porogenic co-solvent and although average binding site affinities were marginally reduced, 2.3×10(4)Lmol(-1)vs. 2.7×10(4)Lmol(-1) measured for a polymer prepared in acetonitrile, these polymers contained the highest number of binding sites, 5.27μmolg(-1)vs. 1.64μmolg(-1), while they also exhibited enhanced selectivity for methylated guanosine derivatives. When applied as sorbents in the extraction of nucleoside derivative cancer biomarkers from synthetic urine samples, significant sample clean-up and recoveries of up to 90% for 7-methylguanosine were achieved.

MATERIALS
Product Number
Brand
Product Description

Supelco
Water, for TOC analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, NMR reference standard
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, ≥98.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, ≥99.9%
Pricing and availability is not currently available.
Supelco
Water, suitable for ion chromatography
Pricing and availability is not currently available.
Supelco
Acrylamide, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylic acid, anhydrous, contains 200 ppm MEHQ as inhibitor, 99%
Pricing and availability is not currently available.
Supelco
Density Standard 998 kg/m3, H&D Fitzgerald Ltd. Quality
Pricing and availability is not currently available.
Sigma-Aldrich
Water, BioPerformance Certified
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, purification grade, 99.8%
Pricing and availability is not currently available.
Supelco
Water, for inorganic trace analysis, ACS reagent, for ultratrace analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, for Northern and Southern blotting, powder blend
Pricing and availability is not currently available.
Sigma-Aldrich
Water, tested according to Ph. Eur.
Pricing and availability is not currently available.
Sigma-Aldrich
Water, for molecular biology, sterile filtered
Pricing and availability is not currently available.
Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
Pricing and availability is not currently available.
Sigma-Aldrich
Cytidine, 99%
Pricing and availability is not currently available.
Supelco
Methanol, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Sodium acrylate, 97%
Pricing and availability is not currently available.
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Water, Nuclease-Free Water, for Molecular Biology
Pricing and availability is not currently available.
Sigma-Aldrich
E-Toxate Water, endotoxin, free
Pricing and availability is not currently available.
Sigma-Aldrich
Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, suitable for electrophoresis, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Acrylamide, for molecular biology, ≥99% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
Water, PCR Reagent
Pricing and availability is not currently available.
Sigma-Aldrich
Cytidine, BioReagent, suitable for cell culture, powder, ≥99%
Pricing and availability is not currently available.
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
Residual Solvent - Acetonitrile(solution in DMSO), Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.