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Safety Information

A3553

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99% (HPLC), powder

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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10 G
CN¥383.71
25 G
CN¥509.14
100 G
CN¥1,768.94

CN¥383.71

Available to ship onApril 10, 2025Details

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10 G
CN¥383.71
25 G
CN¥509.14
100 G
CN¥1,768.94

About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
79-06-1
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
201-173-7
MDL number:
MFCD00008032
UNSPSC Code:
41105319
eCl@ss:
39031205
PubChem Substance ID:
24890783
NACRES:
NB.22

CN¥383.71

Available to ship onApril 10, 2025Details

Request a Bulk Order

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

Assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

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application(s)

pharmaceutical, sterility testing

application(s)

pharmaceutical
sterility testing

application(s)

pharmaceutical
sterility testing

application(s)

pharmaceutical
sterility testing

pore size

0.2 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

packaging

pkg of 25 units

packaging

pkg of 10 blisters per box, Single packed

packaging

pkg of 10 blisters per box, Single packed

packaging

pkg of 10 blisters in 2 bags of 5 blisters per box, Double packed

material

PTFE (hydrophobic membrane filter), stainless steel (for needle), PVC device

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), mixed cellulose esters (MCE) membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), PVDF membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

material

Nylon 66 adapter (for needle), PVC tubing (double lumen), PVDF membrane, plain filter, stainless steel (for needle), styrene-acrylonitrile (SAN) (for canister)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Acrylamide functions as a quencher of tryptophan fluorescence.[1] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[2] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[2] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[1]

Application

Acrylamide has been used in the preparation of polyacrylamide gels.[3][4]

Other Notes

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

suggested gloves for splash protection

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
0000345969
0000338572
0000326888
0000345969
BCCL4597

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The Protein Protocols Handbook (2007)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions
Chiang MYM, et al.
Biomaterials, 34(38), 9754-9762 (2013)
Chemistry, biochemistry, and safety of acrylamide. A review
Friedman M
Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)
I-Chien Liao et al.
Advanced functional materials, 23(47), 5833-5839 (2014-03-01)
The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
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