Skip to Content
Merck
CN
All Photos(1)

Documents

A3574

Sigma-Aldrich

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 29:1

Synonym(s):

30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770837
NACRES:
NA.25

product name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1

sterility

0.2 μm filtered

Quality Level

200

product line

BioReagent

form

liquid

feed ratio

29:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3(22) (2013)
Subcellular western blotting of single cells
Yamauchi KA and Herr AE
Microsystems & Nanoengineering, 3, 16079-16079 (2017)
Ana Techniqs in Biotechnology (2011)
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)
Thi Cuc Mai et al.
Scientific reports, 10(1), 5724-5724 (2020-04-03)
Communication technologies based on radiofrequency (RF) propagation bring great benefits to our daily life. However, their rapid expansion raises concerns about possible impacts on public health. At intensity levels below the threshold to produce thermal effects, RF exposure has also
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service