Skip to Content
Merck
CN
All Photos(1)

Documents

95284

Sigma-Aldrich

Water

for molecular biology, suitable for FISH

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
H2O
Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
12191602
PubChem Substance ID:
57653665
NACRES:
NA.31

grade

for molecular biology

Quality Level

100

sterility

sterile-filtered

form

liquid

quality

sterile filtered

shelf life

limited shelf life, expiry date on the label

technique(s)

FISH: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.34 (lit.)

pH

5-7

conductivity

0.0-5 μS/cm at 25 °C

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

cation traces

Ca: <10 ppb
Mg: <10 ppb

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

  • for cloning and plasmid construction.
  • for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.
  • in RNA isolation.
  • fluorescence in situ hybridization (FISH).
  • to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA.
  • for DNA quantification using quantitative polymerase chain reaction (qPCR).

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia
Fung J, et al.
eLife, 4, e08997-e08997 (2015)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
A guide to transient expression of membrane proteins in HEK-293 cells for functional characterization
Ooi A, et al.
Frontiers in Physiology, 7, 300-300 (2016)
Andreas K Brödel et al.
Nature protocols, 12(9), 1830-1843 (2017-08-11)
Directed evolution is a powerful tool to improve the characteristics of biomolecules. Here we present a protocol for the intracellular evolution of proteins with distinct differences and advantages in comparison with established techniques. These include the ability to select for
Philippe Robert et al.
Investigative radiology, 50(8), 473-480 (2015-06-25)
To prospectively compare in healthy rats the effect of multiple injections of macrocyclic (gadoterate meglumine) and linear (gadodiamide) gadolinium-based contrast agents (GBCAs) on T1-weighted signal intensity in the deep cerebellar nuclei (DCN), including the dentate nucleus. Healthy rats (n =
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service